Collagen-Induced Arthritis Mouse Model.
Collagen-Induced Arthritis Mouse Model.
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DOI：
10.1002/cpz1.313
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发表时间：
2021-12-01
期刊：
影响因子：
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通讯作者：
Brand, David D
中科院分区：
文献类型：
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作者：
Rosloniec, Edward F;Whittington, Karen;Brand, David D
胶原性关节炎小鼠模型是一种被广泛研究的类风湿性关节炎自身免疫模型。在这个模型中，用完全弗氏佐剂乳化的II型胶原免疫遗传易感品系的小鼠，诱导自身免疫性关节炎。本文描述了获取、处理和准备CII的必要步骤，以及选择小鼠品系、适当的免疫技术以及评估自身免疫性关节炎的发病率和严重程度的方法。在这个模型中，关节炎的第一个迹象大约在免疫后21到28天出现。本文中的方案应该为研究人员提供所需的所有必要信息，以在遗传易感品系中重复诱导高发病率的CIA，并对疾病的病理进行批判性评估。出版于2021年。这篇文章是美国政府的工作，属于美国的公共领域。基本方案：诱导胶原诱导的关节炎支持方案1：提纯II型胶原支持方案2：提纯II型胶原α1(II)链支持方案3：评估关节炎的发病率和严重程度支持方案4：通过间接ELISA法测定CII特异性抗体支持方案5：将CII偶联到磁珠支持方案6：通过基于磁珠的EL ISA测定CII特异性抗体支持方案7：测量CIA中T细胞对CII的反应。
The collagen-induced arthritis mouse model is a widely studied autoimmune model of rheumatoid arthritis. In this model, autoimmune arthritis is induced by immunization of genetically susceptible strains of mice with type II collagen emulsified in complete Freund's adjuvant. This article describes the steps necessary for the acquisition, handling, and preparation of CII, in addition to the selection of mouse strains, proper immunization technique, and methods for evaluation of the incidence and severity of the autoimmune arthritis. In this model, the first signs of arthritis appear approximately 21 to 28 days after immunization. The protocols in this article should provide the investigator with all the necessary information required to reproducibly induce a high incidence of CIA in genetically susceptible strains of mice, and to critically evaluate the pathology of the disease. Published 2021. This article is a U.S. Government work and is in the public domain in the USA. Basic Protocol: Induction of collagen-induced arthritis Support Protocol 1: Purification of type II collagen Support Protocol 2: Purification of type II collagen alpha1(II) chains Support Protocol 3: Assessment of arthritis incidence and severity Support Protocol 4: Measurement of CII specific antibody by indirect ELISA Support Protocol 5: Coupling CII to magnetic beads Support Protocol 6: Measuring CII-specific antibody by magnetic-bead based ELISA Support Protocol 7: Measurement of T cell responses to CII in CIA.