THE DEMONSTRATION OF NEW HUMAN BRAIN-SPECIFIC PROTEINS BY HIGH-RESOLUTION TWO-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS
THE DEMONSTRATION OF NEW HUMAN BRAIN-SPECIFIC PROTEINS BY HIGH-RESOLUTION TWO-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS
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DOI：
10.1016/0022-510x(81)90032-0
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发表时间：
1981-01-01
影响因子：
4.4
通讯作者：
THOMPSON, RJ
中科院分区：
文献类型：
--
作者：
JACKSON, P;THOMPSON, RJ
使用高分辨率多重二维聚丙烯酰胺凝胶电泳分析人脑[以及心脏、骨骼肌、子宫、结肠、肺、肝、前列腺、肾、肾上腺、卵巢、睾丸、脾、血浆和溶血物]的可溶性蛋白提取物。大约200种蛋白质基因产物可以在可溶性人脑蛋白质的电泳图谱上可视化。通过将不同人体器官的提取物分别与脑提取物混合，发现了8种蛋白质，它们似乎存在于脑中，其浓度至少比任何其他器官高20倍。其中4个脑特异蛋白与纯化蛋白共电泳鉴定为14-3-2蛋白、肌酸激酶-BB同工酶、醛缩酶C4同工酶和14-3-3蛋白。其余4种蛋白质的身份尚不清楚。
High-resolution multiple 2-dimensional polyacrylamide gel electrophoresis was used to analyze soluble protein extracts from human brain [as well as heart, skeletal muscle, uterus, colon, lung, liver, prostate, kidney, adrenal, ovary, testis, spleen, plasma and hemolysate]. Approximately 200 protein gene products can be visualized on an electrophoretogram of soluble human brain proteins. By electrophoresing extracts of different human organs separately and mixed with brain extract, 8 proteins were found which appear to be present in brain in concentrations at least 20 times greater than in any other organ. Four of these brain-specific proteins were identified by co-electrophoresis with purified proteins as 14-3-2 protein, creatine kinase-BB isoenzyme, aldolase C4 isoenzyme and 14-3-3 protein. The identities of the remaining 4 proteins are unknown.