Expression and purification of non-tagged recombinant mouse SPP1 in E. coli and its biological significance
Expression and purification of non-tagged recombinant mouse SPP1 in E. coli and its biological significance
复制标题
非标记重组小鼠SPP1在大肠杆菌中的表达、纯化及其生物学意义
DOI：
10.4161/bioe.34424
复制
发表时间：
2014-11
期刊：
影响因子：
4.9
通讯作者：
Yunsheng Yuan
中科院分区：
文献类型：
--
作者：
Shengyan Weng;Liang Zhou;Lei Han;Yunsheng Yuan
分泌型磷蛋白1（SPP1）是一种多功能蛋白质，由多种组织的细胞表达。它参与许多生理和病理过程，包括骨代谢、炎症进展、肿瘤转移、损伤修复和高氧诱导的损伤。来自多个物种的天然SPP1已从乳汁和尿液中分离，具有不同标签的重组SPP1已从细菌中表达和纯化。本研究将不含信号肽的小鼠SPP 1基因片段构建到pET 28a（+）载体中，并在大肠杆菌BL21（DE3）中表达了无标签的重组小鼠SPP 1（rmSPP 1）。rmSPP 1的纯化采用一种新的三步法，产品具有高纯度和低内毒素水平。rmSPP 1在体外能有效地促进肝癌细胞（HCC）的增殖，证明其具有生物学活性。
Secreted phosphoprotein 1 (SPP1) is a multifunctional protein expressed by cells from a large variety of tissues. It is involved in many physiological and pathological processes, including bone metabolism, inflammation progress, tumor metastasis, injury repair, and hyperoxia-induced injury. Native SPP1 from multiple species have been isolated from the milk and urine, and recombinant SPP1 with different tags have been expressed and purified from bacteria. In our study, DNA fragments corresponding to mouse SPP1 without signal peptide were built into the pET28a(+) vector, and non-tagged recombinant mouse SPP1 (rmSPP1) was expressed in Escherichia coli BL21(DE3). rmSPP1 was purified using a novel tri-step procedure, and the product features high purity and low endotoxin level. rmSPP1 can effectively increase hepatocellular carcinoma cell (HCC) proliferation in vitro, demonstrating its biological activity.
登录
查看更多内容
影响因子：
13.5
作者：
Ge, Xiaodong;Leung, Tung-Ming;Arriazu, Elena;Lu, Yongke;Urtasun, Raquel;Christensen, Brian;Fiel, Maria Isabel;Mochida, Satoshi;Sorensen, Esben S.;Nieto, Natalia
通讯作者：
Nieto, Natalia
影响因子：
1.6
作者：
S. Sørensen;S. J. Justesen;A. Johnsen
通讯作者：
S. Sørensen;S. J. Justesen;A. Johnsen
DOI：
10.1152/ajpgi.00014.2013
发表时间：
2013-05-01
影响因子：
4.5
作者：
Ge, Xiaodong;Lu, Yongke;Nieto, Natalia
通讯作者：
Nieto, Natalia
影响因子：
11.2
作者：
E. Medico;Alessandra Gentile;C. L. Celso;Tracy Ann Williams;Giovanna Gambarotta;L. Trusolino;P. M. Comoglio
通讯作者：
E. Medico;Alessandra Gentile;C. L. Celso;Tracy Ann Williams;Giovanna Gambarotta;L. Trusolino;P. M. Comoglio
影响因子：
6.9
作者：
Gao, YA;Agnihotri, R;Liaw, L
通讯作者：
Liaw, L