Coal combustion related fine particulate matter (PM2.5) induces toxicity in Caenorhabditis elegans by dysregulating microRNA expression
Coal combustion related fine particulate matter (PM2.5) induces toxicity in Caenorhabditis elegans by dysregulating microRNA expression
复制标题
煤炭燃烧相关的细颗粒物 (PM2.5) 通过 microRNA 表达失调诱导秀丽隐杆线虫毒性
DOI：
10.1039/c7tx00107j
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发表时间：
2017
影响因子：
2.1
通讯作者：
Wang Dayong
中科院分区：
文献类型：
--
作者：
Wu Qiuli;Han Xiaoxiao;Wang Di;Zhao Fang;Wang Dayong
We employed anin vivoassay system ofCaenorhabditis elegansto determine if and which microRNAs (miRNAs) were dysregulated upon exposure to coal combustion related fine particulate matter (PM2.5) by profiling the miRNAs using SOLiD sequencing. From this, expression of 25 miRNAs was discovered to become dysregulated by exposure to PM2.5. Using the correspondingC. elegansdeletion mutants, 5 miRNAs (mir-231,mir-232,mir-230,mir-251andmir-35) were found to be involved in the control of PM2.5toxicity. Furthermore, mutation ofmir-231ormir-232induced a resistance to PM2.5toxicity, whereas mutation ofmir-230,mir-251, ormir-35induced a susceptibility to PM2.5toxicity. SMK-1, an ortholog of the mammalian SMEK protein, was identified as a molecular target formir-231in the regulation of PM2.5toxicity. In addition, the genes ofsod-3,sod-4andctl-3, which are necessary for protection against oxidative stress, were determined to be important downstream targets ofsmk-1in the regulation of PM2.5toxicity. The triggering of thismir-231-SMK-1-SOD-3/SOD-4/CTL-3 signaling pathway may be a critical molecular basis for the role of oxidative stress in the induction of coal combustion related PM2.5toxicity.
