Measuring collagen fiber orientation: A two-dimensional quantitative macroscopic technique
Measuring collagen fiber orientation: A two-dimensional quantitative macroscopic technique
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DOI：
10.1115/1.2798026
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发表时间：
1998-08-01
影响因子：
1.7
通讯作者：
Bednar, DA
中科院分区：
文献类型：
--
作者：
Dickey, JP;Hewlett, BR;Bednar, DA
本文介绍了一种新的系统的设计，评估和应用量化的二维胶原纤维在软组织中的方向。使用定制设计的宏观镜收集一系列透射偏振光图像。结合像素亮度分析，从图像收集与补偿板色调，允许分配到适当的方向带的每个像素。进行实验以量化系统的线性度和噪声。对由不同方向的应变双折射塑料条组成的样本进行了验证。初步胶原纤维取向数据来自肌腱标本。这项研究证明了这种方法的实用性，研究胶原纤维的方向在大面积。
This paper describes the design, evaluation, and application of a new system for quantifying two-dimensional collagen fiber orientation in soft tissue. Series of transmitted polarized light images were collected using a custom-designed macroscope. Combined analysis of pixel brightness, and hue from images collected with a compensator plate, permitted the assignment of each pixel into the appropriate orientation band. Experiments were performed to quantify the linearity and noise of the system. Validation was performed on a specimen composed of strain-birefringent plastic strips at various orientations. Preliminary collagen fiber orientation data is presented from a tendon specimen. This study demonstrates the utility of this approach for studying collagen fiber orientation across large areas.