Automated liquid dispensing pin for DNA microarray applications
Automated liquid dispensing pin for DNA microarray applications
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DOI：
10.1109/tase.2006.871481
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发表时间：
2006-04-01
影响因子：
5.6
通讯作者：
Soteropoulos, P
中科院分区：
文献类型：
--
作者：
Chang, TN;Parthasarathy, S;Soteropoulos, P
本文介绍了一种新的液体分配/抽吸系统，能够产生微米级的斑点/液滴的分子生物学研究和分析。特别是，该应用程序集中在脱氧核糖核酸微阵列制造的目标是均匀的斑点形态，更小的斑点大小，更高的产量，更有效地利用生物材料，和处理高粘度液体的能力。该新系统基于主动传感和控制，是用于基因组和蛋白质组学应用的完全集成的机器人微阵列系统的一部分。该原型系统处理粘稠液体，如100%甘油以及水溶液，并以非接触方式产生均匀的斑点，斑点尺寸范围从80微米到200微米。
This paper describes a new liquid dispensing/aspirating system that is capable of producing micron-size spots/droplets for molecular biology research and analysis. In particular, the application is focused on deoxyribonucleic-acid microarray fabrication with the goals of uniform spot morphology, smaller spot size, higher yield, more efficient use of biological materials, and the capacity to handle high viscosity liquids. The new system is based on active sensing and control and it is part of a fully integrated robotic microarray system for genomic and proteomic applications. The prototype system handles thick liquids such as 100% glycerol as well as aqueous solutions and generates uniform spots in a contactless manner with controllable spot size ranging from 80 microns to 200 microns.