Treatment and risk factor analysis of hypoglycemia in diabetic rhesus monkeys
Treatment and risk factor analysis of hypoglycemia in diabetic rhesus monkeys
复制标题
糖尿病恒河猴低血糖的治疗及危险因素分析
DOI：
10.1258/ebm.2010.010208
复制
发表时间：
2011-02-01
影响因子：
3.2
通讯作者：
Cheng, Jingqiu
中科院分区：
文献类型：
--
作者：
He, Sirong;Chen, Younan;Cheng, Jingqiu
为了预测和及时治疗接受胰岛素或其他降糖药物治疗的糖尿病猴中的低血糖，分析了这些动物中低血糖的发生率和症状之间的关系，以及模型开发和维持中涉及的许多因素。对22只猴子进行不同的程序以诱导糖尿病。通过血糖、胰岛素、C肽水平和静脉葡萄糖耐量试验评价猴模型。对糖尿病猴实施葡萄糖治疗计划，并定期进行实验室检查。建立低血糖治疗的标准程序，并采用Logistic回归模型分析低血糖的危险因素。采用单因素方差分析和t检验，分析糖尿病四种诱导方法与肾功能、血糖控制和低血糖的关系。我们发现糖尿病猴的低血糖状况通过我们的治疗得到迅速改善。统计学分析提示造模方法、肾功能、血糖控制与低血糖发生率有关。具体而言，糖尿病的进展、血糖控制的影响，特别是低血糖的严重程度，根据所用的诱导策略而不同。胰腺部分切除加小剂量链脲佐菌素诱导的模型不容易发生低血糖，血糖控制稳定。然而，全胰切除术诱导的模型更容易发生严重的低血糖，其血糖控制最不稳定。糖尿病发生后血肌酐和甘油三酯水平升高，与低血糖的发生有关。总之，我们认为全胰切除术和肾功能损害是糖尿病猴发生低血糖的两个重要危险因素。应重视糖尿病猴的日常护理，特别是监测和保护其肾功能。
In order to anticipate and promptly treat hypoglycemia in diabetic monkeys treated with insulin or other glucose-lowering drugs, the relationships between the incidence and symptoms of hypoglycemia in these animals, and many factors involved in model development and sustainment were analyzed. Different procedures were performed on 22 monkeys for the induction of diabetes. The monkey models were evaluated by blood glucose, insulin, C-peptide levels and intravenous glucose tolerance tests. A glucose treatment program for the diabetic monkeys was administered and laboratory tests were regularly performed. A standard procedure of hypoglycemia treatment was established and the risk factors of hypoglycemia were analyzed by a logistic regression model. Furthermore, the relationships between the four methods of diabetes induction, renal function, glycemic control and hypoglycemia were studied using one-way analysis of variance and t-test. We found that the hypoglycemic conditions of diabetic monkeys were improved rapidly by our treatment. The statistical analysis suggested that the modeling methods, renal function and glycemic control were related to the incidence of hypoglycemia. In detail, the progress of diabetes, effects of glycemic control and, particularly, the severity of the hypoglycemia differed according to the induction strategy used. The models induced by partial pancreatectomy with low-dose streptozotocin were not prone to hypoglycemia and their glycemic controls were stable. However, the models induced by total pancreatectomy were more vulnerable to severe hypoglycemia and their glycemic controls were the most unstable. Moreover, the levels of blood creatinine and triglyceride increased after the development of diabetes, which was related to the occurrence of hypoglycemia. In conclusion, we suggested that total pancreatectomy and renal impairment are two important risk factors for hypoglycemia in diabetic monkeys. More attention should be paid to daily care of diabetic monkeys, particularly monitoring and protecting their renal function.