p53 protein expression levels as bioindicator of individual exposure to ionizing radiation by flow cytometry
p53 protein expression levels as bioindicator of individual exposure to ionizing radiation by flow cytometry
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DOI：
10.1007/s11010-007-9620-5
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
de Faria Machado, Cintia Gonsalves
中科院分区：
文献类型：
--
作者：
Cavalcanti, Mariana Brayner;Amaral, Ademir de Jesus;de Faria Machado, Cintia Gonsalves
电离辐射（Ionizing radiation，IR）可引起DNA的各种损伤，从而诱导p53表达水平的增加，以修复辐射损伤。因此，p53表达的增加和辐射之间的相关性可能构成一个快速和强大的方法，个别监测的情况下，意外或疑似暴露于IR。在这种情况下，本研究的目的是评估淋巴细胞p53表达水平的变化，基于流式细胞术，在体外照射后的外周血样本。为了测量p53蛋白的这种表达水平，进行了研究以建立基于流式细胞术的分析方法。因此，p53蛋白的表达水平与吸收剂量之间的关系已经得到验证。本报告中提出的结果强调，流式细胞术作为一个重要的工具，快速评价p53蛋白表达水平的生物指标，个人暴露于急性电离辐射。
Ionizing radiation (IR) can cause various lesions in DNA, which induce the increase of p53 expression levels in order to repair radiation induced damage. Thus, the correlation between the increase of p53 expression and an irradiation may constitute a fast and powerful method of individual monitoring in cases of accidental or suspected exposures to IR. In this context, the aim of this research was to evaluate changes in lymphocyte p53 expression levels, based on flow cytometry, after in vitro irradiation of peripheral blood samples. For the measurement of such expression levels of p53 protein, an investigation was carried out in order to establish a methodology of analysis based on flow cytometry. Hence, relationships among levels of expression of p53 protein with the absorbed dose have been verified. The results presented in this report emphasized flow cytometry as an important tool for the fast evaluation of p53 protein expression levels as bioindicator of individual exposure to acute ionizing radiation.