Enhancer-trap flippase lines for clonal analysis in the Drosophila ovary.
Enhancer-trap flippase lines for clonal analysis in the Drosophila ovary.
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DOI：
10.1534/g3.114.010710
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发表时间：
2014-07-14
期刊：
影响因子：
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通讯作者：
Nystul T
中科院分区：
文献类型：
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作者：
Huang P;Sahai-Hernandez P;Bohm RA;Welch WP;Zhang B;Nystul T
黑腹果蝇遗传工具箱包括许多用于产生遗传镶嵌组织的原种，其中通过谱系相关的细胞克隆包含共同的遗传改变。这些工具使得研究必需基因的胚后功能成为可能，并更好地了解单个细胞如何在完整组织中相互作用。我们已经通过201增强子陷阱翻转酶线筛选，以确定线，产生有用的克隆模式，在成年卵巢。我们发现，大约70%的线产生的克隆，存在于成年卵巢和许多卵巢细胞类型的代表之间的不同克隆模式产生的这些线。我们还确定并进一步表征了五个特别有用的增强子陷阱翻转酶系。这些细胞系使得有可能在生殖细胞、陪伴细胞、卵泡前细胞或终丝细胞中产生特异性克隆。此外，我们还发现山雀在后护航细胞、卵泡干细胞和卵泡前细胞中特异性上调，这些细胞包括卵泡干细胞龛区域。总的来说，这些研究为果蝇卵巢的遗传镶嵌分析提供了几种新的工具。
The Drosophila melanogaster genetic tool box includes many stocks for generating genetically mosaic tissue in which a clone of cells, related by lineage, contain a common genetic alteration. These tools have made it possible to study the postembryonic function of essential genes and to better understand how individual cells interact within intact tissues. We have screened through 201 enhancer-trap flippase lines to identify lines that produce useful clone patterns in the adult ovary. We found that approximately 70% of the lines produced clones that were present in the adult ovary and that many ovarian cell types were represented among the different clone patterns produced by these lines. We have also identified and further characterized five particularly useful enhancer-trap flippase lines. These lines make it possible to generate clones specifically in germ cells, escort cells, prefollicle cells, or terminal filament cells. In addition, we have found that chickadee is specifically upregulated in the posterior escort cells, follicle stem cells, and prefollicle cells that comprise the follicle stem cell niche region. Collectively, these studies provide several new tools for genetic mosaic analysis in the Drosophila ovary.