Prevalence of aerobic bacteria in bronchoalveolar lavage fluids from healthy pigs.
Prevalence of aerobic bacteria in bronchoalveolar lavage fluids from healthy pigs.
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健康猪支气管肺泡灌洗液中需氧细菌的患病率。
DOI：
10.2460/ajvr.1994.55.12.1697
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发表时间：
1994
影响因子：
1
通讯作者：
Klaus Petzoldt
中科院分区：
文献类型：
--
作者：
Andreas Hensel;Martin Ganter;Sabine Kipper;Silvester Krehon;Max M. Wittenbrink;Klaus Petzoldt
在猪中进行纤维支气管镜检查，以评估通过使用该方法获得的支气管肺泡灌洗液（BALF）的细菌污染，并确定健康猪支气管肺泡气道中的需氧菌种类。使用显色细菌示踪菌株对插入支气管镜引起的BALF细菌污染进行了评价，发现其占总菌落形成单位（CFU）的0.22%，范围为0 - 1.6%。从6个封闭猪群中选择164头肺健康猪。对从这些猪中获得的BALF进行细菌学检查。10.4%的BALF不能分离出细菌; 5.5%的BALF样本产生纯培养物; 84.1%产生混合需氧细菌生长。在29.2%猪的BALF中，分离出≤ 5 × 10（2）CFU/ml的细菌。50%猪的BALF中细菌总数在5 × 10（2）和10（3）CFU/ml之间变化; 10.4%的BALF样本含有10（3）CFU/ml和5 × 10（3）CFU/ml。84.1%的猪一次肺灌洗液中分离到1种以上的细菌。从单个BALF样品中分离出多达6种。共分离出443株细菌，分为25个属和种。BALF样本产生葡萄球菌（67.6%：猪葡萄球菌占样本的13.4%，金黄色葡萄球菌占2.4%）、α-溶血性链球菌（49.4%）、大肠埃希菌（42.1%）、非溶血性链球菌（26.2%）、克雷伯菌属（18.3%）、微球菌（12.8%）和棒状杆菌（11.0%）。也发现了其他细菌物种，但频率较低。（250字处删节）
Fiberoptic bronchoscopy was performed in pigs to assess bacterial contamination of bronchoalveolar lavage fluids (BALF) obtained by use of the method and to determine the aerobic bacterial species in bronchoalveolar airways of healthy pigs. Bacterial contamination of BALF caused by insertion of the bronchoscope was evaluated, using a chromogenic bacterial tracer strain, and was found to be 0.22% of total colony-forming units (CFU), with range between 0 and 1.6%. A total of 164 pulmonary-healthy pigs from 6 closed herds were selected. The BALF obtained from these pigs were examined bacteriologically. Bacteria could not be isolated from 10.4% of all BALF; 5.5% of the BALF samples yielded pure cultures; and 84.1% yielded mixed aerobic bacterial growth. In BALF from 29.2% of the pigs, < or = 5 x 10(2) CFU of bacteria/ml were isolated. The total number of bacteria in BALF from 50% of the pigs varied between 5 x 10(2) and 10(3) CFU/ml; 10.4% of BALF samples contained between 10(3) CFU/ml and 5 x 10(3) CFU/ml. More than 1 bacterial species were isolated from a single lung lavage of 84.1% of the pigs. Up to 6 species were isolated from a single BALF sample. A total of 443 bacterial isolates were differentiated into 25 bacterial genera and species. Samples of BALF yielded staphylococci (67.6%: Staphylococcus hyicus from 13.4% of the samples and S aureus from 2.4%), alpha-hemolytic streptococci (49.4%), Escherichia coli (42.1%), non-hemolytic streptococci (26.2%), Klebsiella spp (18.3%), micrococci (12.8%), and Coryneformes (11.0%). Other bacterial species were found, but less frequently.(ABSTRACT TRUNCATED AT 250 WORDS)