Biosensing the acute toxicity of metal interactions: Are they additive, synergistic, or antagonistic?
Biosensing the acute toxicity of metal interactions: Are they additive, synergistic, or antagonistic?
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DOI：
10.1002/etc.5620190332
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发表时间：
2000-03
影响因子：
4.1
通讯作者：
S. Preston;N. Coad;J. Townend;K. Killham;G. Paton
中科院分区：
文献类型：
--
作者：
S. Preston;N. Coad;J. Townend;K. Killham;G. Paton
使用来自不同生态位的两种基于发光的微生物传感器（大肠杆菌 HB101 pUCD607 和荧光假单胞菌 10586 pUCD607）评估了单独或组合的 Zn、Cu 和 Cd 的毒性。锌和铜以及锌和镉组合的毒性作用与大肠杆菌的反应之间发生显着的协同相互作用（即污染物组合的毒性大于单个毒性相加的预测）。镉和铜组合的毒性与荧光假单胞菌的反应之间也观察到显着的协同相互作用。较长的暴露时间被证明可以显着增加大肠杆菌对金属污染物的敏感性。据观察，随着暴露时间的延长，荧光假单胞菌对 Zn 和 Cd 的敏感性显着降低。结果表明，金属组合的毒性不能基于它们的毒性作用彼此独立来建模。讨论了应用不同模型来描述金属组合之间的相互作用。强调了考虑毒性评估所选择的测试物种和暴露时间的重要性，以及进一步研究污染物组合的毒性的必要性。
The toxicity of Zn, Cu, and Cd, alone or in combination, was assessed using two luminescence‐based microbialbiosensors from different ecological niches: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586 pUCD607. Significant synergistic interactions occurred between the toxic effects of the Zn and Cu and Zn and Cd combinations and the response of E. coli (i.e., toxicities of combinations of pollutants were greater than predicted from addition of individual toxicities). Significant synergistic interactions were also observed between the toxicities of Cd and Cu combinations and the response of P. fluorescens. A longer exposure time was shown to significantly increase the sensitivity of E. coli to the metal pollutants. P. fluorescens was observed to significantly decrease in its sensitivity toward Zn and Cd with a longer exposure time. It was shown that the toxicity of combinations of metals could not be modeled on the basis that their toxic action was independent of each other. The application of different models to describe interactions between combinations of metals is discussed. The importance of considering the test species and the exposure period selected for toxicity assessment was highlighted, as was the need to further investigate the toxicity of combinations of pollutants.