The oxidative stress and antioxidant responses of Litopenaeus vannamei to low temperature and air exposure
The oxidative stress and antioxidant responses of Litopenaeus vannamei to low temperature and air exposure
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DOI：
10.1016/j.fsi.2017.11.016
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发表时间：
2018-01-01
影响因子：
4.7
通讯作者：
Mao, Linchun
中科院分区：
文献类型：
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作者：
Xu, Zihan;Regenstein, Joe M.;Mao, Linchun
低温和空气暴露是鱼虾无水运输的关键因素。为了研究南美白对虾在模拟无水运输中的氧化应激和抗氧化反应，将活虾在13℃下冷却3min，在15℃氧气中贮藏12h，然后在25℃水中复苏，在这种无水运输系统下，对虾的存活率达到86.67%以上。观察肝胰腺细胞超微结构的变化，同时测定血淋巴和肝胰腺中超氧化物歧化酶(SOD)、过氧化氢酶(POD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、抗超氧阴离子自由基(ASAFR)、总抗氧化能力(TAOC)、活性氧(ROS)生成量、丙二醛(MDA)含量以及CAT和GSH-PX的相对mRNA表达。冷休克复苏后，肝胰腺细胞中某些细胞器的轻微扭曲是可逆的。SOD、POD、CAT、GSH-Px、TAOC、ROS产生及CAT、GSH-PX相对mRNA的表达均随冷休克时间的延长而升高，在贮藏3h或6h时达到峰值，然后逐渐下降。新鲜对虾和复活对虾的SOD、POD、GSH-Px、TAOC、ROS、MDA及CAT、GSH-Px的相对mRNA表达差异均无统计学意义。氧化应激和抗氧化反应是组织特异性的，因为根据SOD活性、MDA含量和GSH-Px mRNA表达的结果，肝胰腺似乎具有更强的防御细胞器损伤的能力，并且比血淋巴对应激更敏感。这些结果表明，低温和空气暴露导致了显著的氧化和抗氧化反应，但不会导致这个无水系统的不可逆转的损害。
Low temperature and air exposure were the key attributes for waterless transportation of fish and shrimp. In order to investigate the oxidative stress and antioxidant responses of the live shrimp Litopenaeus vannamei in the mimic waterless transportation, live shrimp were cooled at 13 degrees C for 3 min, stored in oxygen at 15 degrees C for 12 h, and then revived in water at 25 degrees C. The survival rate of shrimp under this waterless transportation system was over 86.67%. The ultrastructure of hepatopancreas cells were observed while activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione peroxidase (GSH-Px), antisuperoxide anion free radicals (ASAFR), total antioxidant capacity (TAOC), reactive oxygen species (ROS) production, content of malondialdehyde (MDA) and relative mRNA expressions of CAT and GSH-Px in the hemolymph and hepatopancreas were determined. Slight distortions of some organelles in hepatopancreas cells was reversible upon the shrimp revived from the cold shock. The activities of SOD, POD, CAT, GSH-Px, TAOC, ROS production and relative mRNA expressions of CAT and GSH-Px increased following the cold shock and reached peak levels after 3 or 6 h of storage, and then decreased gradually. There was no significant difference between the fresh and the revived shrimp in SOD, POD, GSH-Px, TAOC, ROS, MDA and relative mRNA expressions of CAT and GSH-Px. The oxidative stress and antioxidant responses were tissue-specific because hepatopancreas seemed to have a greater ability to defend against organelle damage and was more sensitive to stress than hemolymph based on the results of SOD activity, MDA content and GSH-Px mRNA expression. These results revealed that low temperature and air exposure caused significant oxidative and antioxidant responses, but did not lead to irreversible damages in this waterless system.