Tumorsphere as an effective in vitro platform for screening anti-cancer stem cell drugs.
Tumorsphere as an effective in vitro platform for screening anti-cancer stem cell drugs.
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DOI：
10.18632/oncotarget.6261
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发表时间：
2016-01-12
期刊：
影响因子：
--
通讯作者：
Chang WW
中科院分区：
文献类型：
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作者：
Lee CH;Yu CC;Wang BY;Chang WW
癌症干细胞（cancer stem cells，CSC）是癌症组织中具有肿瘤发生、耐药性和转移特性的细胞亚群。CSC也被认为是癌症复发的主要原因。靶向CSC已被认为是成功治疗癌症的关键。肿瘤球培养是基于在补充有生长因子如表皮生长因子和碱性成纤维细胞生长因子的无血清培养基中将癌细胞培养到超低附着表面上。肿瘤球培养被广泛用于分析CSC的自我更新能力，并从大量癌细胞中富集这些细胞。该方法也为筛选潜在的抗CSC药物提供了可靠的平台。与一般单层培养相比，从肿瘤球试验中选择的潜在药物的体外抗增殖活性更容易转化为体内抗肿瘤活性。肿瘤球测定还可以测量潜在抗癌剂的临床试验结果。此外，肿瘤球检测可能是未来癌症治疗创新的一个有前途的策略，并可能有助于抗癌小分子化学物质的筛选。
Cancer stem cells (CSCs) are a sub-population of cells within cancer tissues with tumor initiation, drug resistance and metastasis properties. CSCs also have been considered as the main cause of cancer recurrence. Targeting CSCs have been suggested as the key for successful treatment against cancer. Tumorsphere cultivation is based on culturing cancer cells onto ultralow attachment surface in serum-free media under the supplementation with growth factors such as epidermal growth factor and basic fibroblast growth factor. Tumorsphere cultivation is widely used to analyze the self-renewal capability of CSCs and to enrich these cells from bulk cancer cells. This method also provides a reliable platform for screening potential anti-CSC agents. The in vitro anti-proliferation activity of potential agents selected from tumorsphere assay is more translatable into in vivo anti-tumorigenic activity compared with general monolayer culture. Tumorsphere assay can also measure the outcome of clinical trials for potential anti-cancer agents. In addition, tumorsphere assay may be a promising strategy in the innovation of future cancer therapeutica and may help in the screening of anti-cancer small-molecule chemicals.