Use of Hydrolysates from Yellowfin Tuna (Thunnus albacares) Heads as a Complex Nitrogen Source for Lactic Acid Bacteria
Use of Hydrolysates from Yellowfin Tuna (Thunnus albacares) Heads as a Complex Nitrogen Source for Lactic Acid Bacteria
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DOI：
10.1007/s11947-009-0225-8
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发表时间：
2012-01-01
影响因子：
5.6
通讯作者：
Rasco, Barbara
中科院分区：
文献类型：
--
作者：
Safari, Reza;Motamedzadegan, Ali;Rasco, Barbara
通过酶解黄鳍金枪鱼（Thunnus albacares）鱼头废物得到的两种不同的胨已被证明对乳酸菌（保加利亚乳杆菌波斯型培养收集（PTCC） 1332、嗜酸乳杆菌PTCC 1643、干酪乳杆菌PTCC 1608、德布鲁氏乳杆菌PTCC 1333、植物乳杆菌PTCC 1058、乳酸乳球菌PTCC 1336和酒井乳杆菌PTCC 1712）的生长有有效的促进作用。用Alcalase或Protamex酶解得到的蛋白胨代替商业MRS培养基中使用的标准蛋白胨。Alcalase和Protamex产生的蛋白胨水解度分别为34%和19%。结果表明，Alcalase和Protamex的蛋白胨对乳酸菌（LAB）生长的促进作用优于MRS商品化培养基（P < 0.05）。在最大生长速率和生物量产量方面，用于生产鱼类水解物的蛋白水解酶的选择对所得水解物的性能有相当大的影响。与使用Protamex相比，使用Alcalase产生的蛋白胨具有更高的水解程度，可以诱导更好的生长，并且作为LAB底物的总体性能更好。目前的研究表明，酶修饰的鱼副产品可以作为细菌生长的低投氮源。
Two different peptones obtained by enzymatic hydrolysis of yellowfin tuna (Thunnus albacares) head waste have been shown to be effective in promoting the growth of lactic acid bacteria (Lactobacillus bulgaricus Persian Type Culture Collection (PTCC) 1332, Lactobacillus acidophilus PTCC 1643, Lactobacillus casei PTCC 1608, Lactobacillus delbrukii PTCC 1333, Lactobacillus plantarum PTCC 1058, Lactococcus lactis PTCC 1336, and Lactobacillus sakei PTCC 1712). Peptones obtained from the enzymatic hydrolysis with Alcalase or Protamex were used instead of the standard peptones used in commercial MRS media. Peptones produced by Alcalase and Protamex had a 34% and 19% degree of hydrolysis, respectively. The results showed that the peptones from Alcalase and Protamex were better at promoting lactic acid bacteria (LAB) growth than the commercial MRS media (P < 0.05). The choice of proteolytic enzyme used to produce the fish hydrolysate had a considerable impact on the performance of the resulting hydrolysate, both in terms of maximum growth rate and biomass production. Peptones produced using Alcalase, with a higher degree of hydrolysis, induced better growth and performed better overall as an LAB substrate than those using Protamex. Current study revealed that enzymatic-modified fish by-products can be used as low cast nitrogen source for bacterial growth.