Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression
Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression
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DOI：
10.1038/s41467-020-14774-3
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发表时间：
2020-02-28
影响因子：
16.6
通讯作者：
Tan, Bo
中科院分区：
文献类型：
--
作者：
Ganesh, Swarna;Venkatakrishnan, Krishnan;Tan, Bo
肿瘤干细胞(CSC)可以通过其基因组DNA的修饰来鉴定。在这里，我们报告了一个概念，精确收缩有机半导体表面增强拉曼散射(SERS)探测器到量子尺寸，以研究CSC的表观遗传学轮廓。该探针用于无标签基因组DNA检测，这是一种促进单分子DNA检测的方法。该传感器在一次测试中同时检测到了毫微摩尔浓度中基因组DNA的结构、分子和基因表达的异常。除了指出癌和非癌细胞基因组DNA的差异外，量子尺度有机半导体还能够追踪两个经常被用作CSC标记的基因的表达。量子尺度有机半导体有望成为一种无标记、超灵敏的多重基因组分析的新工具。有机半导体的低检测灵敏度限制了它们在生物医学表面增强拉曼散射中的应用。在这里，作者使用量子规模的有机半导体，并展示了对基因组DNA甲基化和基因表达的检测。
Cancer stem cells (CSC) can be identified by modifications in their genomic DNA. Here, we report a concept of precisely shrinking an organic semiconductor surface-enhanced Raman scattering (SERS) probe to quantum size, for investigating the epigenetic profile of CSC. The probe is used for tag-free genomic DNA detection, an approach towards the advancement of single-molecule DNA detection. The sensor detected structural, molecular and gene expression aberrations of genomic DNA in femtomolar concentration simultaneously in a single test. In addition to pointing out the divergences in genomic DNA of cancerous and non-cancerous cells, the quantum scale organic semiconductor was able to trace the expression of two genes which are frequently used as CSC markers. The quantum scale organic semiconductor holds the potential to be a new tool for label-free, ultra-sensitive multiplexed genomic analysis. The low detection sensitivity of organic semiconductors has limited their use in biomedical surface-enhanced Raman scattering applications. Here, the authors use quantum scale organic semiconductors and show detection of genomic DNA methylation as well as gene expression.