The stable and permanent expansion of functional T lymphocytes in athymic nude rats after a single injection of mature T cells.
The stable and permanent expansion of functional T lymphocytes in athymic nude rats after a single injection of mature T cells.
复制标题
单次注射成熟T细胞后，无胸腺裸鼠中功能性T淋巴细胞的稳定和永久扩增。
DOI：
10.4049/jimmunol.139.5.1379
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发表时间：
1987
影响因子：
4.4
通讯作者：
W. Ford
中科院分区：
文献类型：
--
作者：
E. Bell;S. Sparshott;M. Drayson;W. Ford
胸腺裸大鼠（PVG.rnu/rnu）在6 ~ 10周龄时注射1 ~ 2亿个胸管淋巴细胞（TDL），其中含有40% ~ 60%的成熟T细胞。此后，对注射TDL的裸受体进行长达2年的T细胞功能监测。TDL转移后，淋巴结（LN）中的W3/25+ T辅助细胞（Th）从2周的7%增加到8周的30%。W3/25+细胞的百分比在受体的生命中（长达2年）保持升高，接近正常水平。注射tdl的裸大鼠的循环池总大小在16周时扩大到正常对照组的2/3，在W3/25+细胞中增加了10- 15倍。W3/25+种群的扩张与TDL初始剂量无关。随着时间的推移，脾脏和LN获得了正常的组织学外观，包括生发中心的发育和T细胞交通区细胞数量的显著增加。注射tdl的裸大鼠对同种异体皮肤移植的排斥动力学接近正常。此外，通过腘窝LN试验评估的移植物对宿主（GVH）反应性，在置换后9个月至1年逐渐增加，达到与对照组GVH活性相似的水平。
Athymic nude rats (PVG.rnu/rnu) were injected at 6 to 10 wk of age with 1 to 200 million thoracic duct lymphocytes (TDL) containing 40 to 60% mature T cells. Thereafter TDL-injected nude recipients were monitored for evidence of T cell function for up to 2 yr. W3/25+ T helper (Th) cells in lymph nodes (LN) increased from 7% at 2 wk to 30% at 8 wk after TDL transfer. The percent of W3/25+ cells remained elevated for the life of the recipient (up to 2 yr), approximating normal levels. The total size of the recirculating pool expanded in TDL-injected nude rats to reach 2/3 the level of euthymic controls by 16 wk, an increase of 10-fold to 15-fold in W3/25+ cells. The expansion of the W3/25+ population was independent of initial TDL dose. With time spleen and LN acquired a normal histological appearance including the development of germinal centres and a marked increase in cellularity in T cell traffic areas. TDL-injected nude rats rejected skin allografts with near normal kinetics. In addition graft vs host (GVH) responsiveness, assessed by the popliteal LN assay, progressively increased reaching a level 9 mo to 1 yr after replacement that resembled the GVH activity in euthymic controls.