Lipase Production by Endophytic Fungus Cercospora kikuchii: Stability of Enzymatic Activity after Spray Drying in the Presence of Carbohydrates
Lipase Production by Endophytic Fungus Cercospora kikuchii: Stability of Enzymatic Activity after Spray Drying in the Presence of Carbohydrates
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DOI：
10.1080/07373937.2011.573153
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Said, Suraia
中科院分区：
文献类型：
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作者：
Costa-Silva, Tales A.;Nogueira, Marco A.;Said, Suraia
目前的工作涉及改进菊地尾孢脂肪酶的生产和稳定性。在补充有 2% 大豆油的培养基中 6 天后获得最大酶产量（9.384 U/ml）。将脂肪酶与不同助剂一起喷雾干燥，并研究其稳定性。用 10% (w/v) 乳糖、b-环糊精、麦芽糖糊精、甘露醇、阿拉伯树胶和海藻糖干燥后，残留酶活性范围为 63% 至 100%。在没有佐剂的情况下酶活性丧失。 8 个月后，所使用的大多数佐剂在 5 摄氏度下保持至少 50% 的酶活性，在 25 摄氏度下保持至少 40% 的酶活性。用10%的β-环糊精干燥的脂肪酶在5℃下保留了72%的活性。通过丁基琼脂糖柱将脂肪酶分成4个池，并且池4被部分纯化（33.1%；269.5 U/mg蛋白质）。该池还在麦芽糖糊精 DE10 中喷雾干燥，并且保持了 100% 的活性。
The present work deals with improving the production and stabilization of lipases from Cercospora kikuchii. Maximum enzyme production (9.384 U/ml) was obtained after 6 days in a medium supplemented with 2% soybean oil. The lipases were spray dried with different adjuvants, and their stability was studied. The residual enzyme activity after drying with 10% (w/v) of lactose, b- cyclodextrin, maltodextrin, mannitol, gum arabic, and trehalose ranged from 63 to 100%. The enzyme activity was lost in the absence of adjuvants. Most of the adjuvants used kept up at least 50% of the enzymatic activity at 5 degrees C and 40% at 25 degrees C after 8 months. The lipase dried with 10% of beta-cyclodextrin retained 72% of activity at 5 degrees C. Lipases were separated by butyl-sepharose column into 4 pools, and pool 4 was partially purified (33.1%; 269.5 U/mg protein). This pool was also spray dried in maltodextrin DE10, and it maintained 100% of activity.