Telomerase-mediated telomere addition in vivo requires DNA primase and DNA polymerases α and δ
Telomerase-mediated telomere addition in vivo requires DNA primase and DNA polymerases α and δ
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DOI：
10.1016/s0092-8674(00)81670-0
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发表时间：
1999-12-23
期刊：
影响因子：
64.5
通讯作者：
Gottschling, DE
中科院分区：
文献类型：
--
作者：
Diede, SJ;Gottschling, DE
为了更好地了解体内端粒酶介导的端粒添加的要求，我们在S。酿酒酵母的一种，其产生紧邻短端粒DNA束的染色体末端。从头端作为端粒：它被保护免于以CDC13依赖的方式降解，端粒序列被有效地添加，并且在具有长端粒的突变株中以更快的速率添加。在M期阻滞的细胞中检测到端粒增加，这使我们能够确定必需的DNA聚合酶α和δ以及DNA引发酶是必需的。这表明端粒酶的端粒DNA合成与相反链的产生密切相关。这种协调可以防止端粒酶产生过长的单链尾，这可能对S.啤酒。
To better understand the requirements for telomerase-mediated telomere addition in vivo, we developed an assay in S. cerevisiae that creates a chromosome end immediately adjacent to a short telomeric DNA tract. The de novo end acts as a telomere: it is protected from degradation in a CDC13-dependent manner, telomeric sequences are added efficiently, and addition occurs at a faster rate in mutant strains that have long telomeres. Telomere addition was detected in M phase arrested cells, which permitted us to determine that the essential DNA polymerases alpha and delta and DNA primase were required. This indicates that telomeric DNA synthesis by telomerase is tightly coregulated with the production of the opposite strand. Such coordination prevents telomerase from generating excessively long single-stranded tails, which may be deleterious to chromosome stability in S. cerevisiae.