The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes δ-tubulin, a new member of the tubulin superfamily
The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes δ-tubulin, a new member of the tubulin superfamily
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DOI：
10.1091/mbc.9.6.1293
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发表时间：
1998-06-01
影响因子：
3.3
通讯作者：
Trabuco, EC
中科院分区：
文献类型：
--
作者：
Dutcher, SK;Trabuco, EC
我们克隆了衣藻的UNI3基因，发现它编码了微管蛋白超家族的一个新成员。尽管Uni3p与α -、β -和γ -微管蛋白具有显著的序列一致性，但Uni3p的一个区域与微管蛋白或其他已知蛋白没有相似性。突变的uni3-1细胞聚集0、1或2根鞭毛。系谱分析表明，uni3-1细胞的鞭毛数是细胞年龄的函数。单鞭毛虫的uni3-1细胞表现为定位表型；眼斑对面的基体模板为单鞭毛。有一定比例的uni -1细胞也不能正确地定位卵裂沟，而在衣藻中，卵裂沟的位置与基体有关。最后，在电镜下观察单-1细胞时，在基底体近端观察到双微管而不是三微管。我们认为Uni3微管蛋白参与了基底体/中心粒的功能和细胞周期依赖性成熟。
We have cloned the UNI3 gene in Chlamydomonas and find that it encodes a new member of the tubulin superfamily. Although Uni3p shares significant sequence identity with alpha-, beta-, and gamma-tubulins, there is a region of Uni3p that has no similarity to tubulins or other known proteins. Mutant uni3-1 cells assemble zero, one, or two flagella. Pedigree analysis suggests that flagellar number in uni3-1 cells is a function of the age of the cell. The uniflagellate uni3-1 cells show a positional phenotype; the basal body opposite the eyespot templates the single flagellum. A percentage of uni3-1 cells also fail to orient the cleavage furrow properly, and basal bodies have been implicated in the placement of cleavage furrows in Chlamydomonas. Finally when uni3-1 cells are observed by electron microscopy, doublet rather than triplet microtubules are observed at the proximal end of the basal bodies. We propose that the Uni3 tubulin is involved in both the function and cell cycle-dependent maturation of basal bodies/centrioles.