Optimally chirped multimodal CARS microscopy based on a single Ti: sapphire oscillator
Optimally chirped multimodal CARS microscopy based on a single Ti: sapphire oscillator
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DOI：
10.1364/oe.17.002984
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发表时间：
2009-02-16
期刊：
影响因子：
3.8
通讯作者：
Stolow, Albert
中科院分区：
文献类型：
--
作者：
Pegoraro, Adrian F.;Ridsdale, Andrew;Stolow, Albert
我们展示了高性能的相干反斯托克斯拉曼散射（汽车）显微镜的活细胞和组织与用户可变的光谱分辨率和广泛的拉曼可调谐性（2500 - 4100 cm（-1）），使用飞秒钛：蓝宝石泵和光子晶体光纤输出的宽带同步斯托克斯脉冲。飞秒激光脉冲的光谱啁啾是一个用户变量参数，用于优化多模汽车显微镜的光谱聚焦实现。在2-8 μ s的像素停留时间和低激光功率（总功率< 30 mW）下实现了活细胞和组织的高信噪比、高对比度多模态成像。(c)2009年美国光学学会
We demonstrate high performance coherent anti-Stokes Raman scattering ( CARS) microscopy of live cells and tissues with user-variable spectral resolution and broad Raman tunability ( 2500 - 4100 cm(-1)), using a femtosecond Ti: Sapphire pump and photonic crystal fiber output for the broadband synchronized Stokes pulse. Spectral chirp of the fs laser pulses was a user-variable parameter for optimization in a spectral focussing implementation of multimodal CARS microscopy. High signal-to-noise, high contrast multimodal imaging of live cells and tissues was achieved with pixel dwell times of 2-8 mu s and low laser powers (< 30 mW total). (c) 2009 Optical Society of America