Anaplasma phagocytophilum induces Ixodes scapularis ticks to express an antifreeze glycoprotein gene that enhances their survival in the cold
Anaplasma phagocytophilum induces Ixodes scapularis ticks to express an antifreeze glycoprotein gene that enhances their survival in the cold
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DOI：
10.1172/jci42868
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发表时间：
2010-09-01
影响因子：
15.9
通讯作者：
Fikrig, Erol
中科院分区：
文献类型：
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作者：
Neelakanta, Girish;Sultana, Hameeda;Fikrig, Erol
在美国，肩突硬蜱在东北部和上中西部越冬，并传播人类粒细胞无形体病的病原体，嗜吞噬细胞无形体，以及其他病原体。我们现在证明了A.嗜吞噬细胞I.肩胛蜱增加了它们在寒冷中生存的能力。我们发现了一个I。肩胛肌抗冻糖蛋白，命名为IAFGP，并通过RNAi敲除研究证明了IAFGP对I.肩胛骨蜱在寒冷的环境。转染研究还表明，IAFGP增加了低温下酵母细胞的活力。值得注意的是，A.嗜吞噬细胞菌可诱导Iafgp的表达，从而提高Iafgp的耐寒性和存活率。肩胛肌这些数据定义了人类致病细菌及其节肢动物载体之间共生的分子基础，并描绘了我们认为可能有针对性地改变这种微生物及其无脊椎动物宿主的生命周期的新途径。
In the United States, Ixodes scapularis ticks overwinter in the Northeast and Upper Midwest and transmit the agent of human granulocytic anaplasmosis, Anaplasma phagocytophilum, among other pathogens. We now show that the presence of A. phagocytophilum in I. scapularis ticks increases their ability to survive in the cold. We identified an I. scapularis antifreeze glycoprotein, designated IAFGP, and demonstrated via RNAi knockdown studies the importance of IAFGP for the survival of I. scapularis ticks in a cold environment. Transfection studies also show that IAFGP increased the viability of yeast cells subjected to cold temperature. Remarkably, A. phagocytophilum induced the expression of iafgp, thereby increasing the cold tolerance and survival of I. scapularis. These data define a molecular basis for symbiosis between a human pathogenic bacterium and its arthropod vector and delineate what we believe to be a new pathway that may be targeted to alter the life cycle of this microbe and its invertebrate host.