Analysis of the complete plastid genome of the unicellular red alga Porphyridium purpureum
Analysis of the complete plastid genome of the unicellular red alga Porphyridium purpureum
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DOI：
10.1007/s10265-014-0627-1
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发表时间：
2014-03
影响因子：
2.8
通讯作者：
Naoyuki Tajima;Shusei Sato;F. Maruyama;K. Kurokawa;H. Ohta;S. Tabata;Kohsuke Sekine;Takashi Moriyama;N. Sato
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文献类型：
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作者：
Naoyuki Tajima;Shusei Sato;F. Maruyama;K. Kurokawa;H. Ohta;S. Tabata;Kohsuke Sekine;Takashi Moriyama;N. Sato
我们测定了单细胞海洋红藻紫花卟啉（porphyridium purpureumstrain NIES 2140）质体基因组的完整核苷酸序列，该菌株属于未测序的卟啉类。基因组为217,694 bp的环状DNA， GC含量为30.3%。224个蛋白质编码基因中有29个含有一个或多个内含子。在pl28基因中发现了I组内含子，而其他内含子为II组内含子。就会。紫瘤质体基因组有1个非编码RNA （ncRNA）基因、29个tRNA基因和2个不相同的核糖体RNA操纵子。一个rRNA操纵子在其和其基因之间有一个tRNAAla（UGC）基因，而另一个rRNA操纵子有一个tRNAIle（GAU）基因。系统发育分析表明，异厚藻、隐藻和附藻的质体起源于红藻亚门。p的核糖体蛋白簇的基因顺序。紫红色质体基因组不同于其他红水藻和染色水藻。这些结果表明，p的质体基因组发生了大规模的重排。从其它红藻分离后的紫藻。
We determined the complete nucleotide sequence of the plastid genome of the unicellular marine red algaPorphyridium purpureumstrain NIES 2140, belonging to the unsequenced class Porphyridiophyceae. The genome is a circular DNA composed of 217,694 bp with the GC content of 30.3 %. Twenty-nine of the 224 protein-coding genes contain one or multiple intron(s). A group I intron was found in therpl28gene, whereas the other introns were group II introns. TheP. purpureumplastid genome has one non-coding RNA (ncRNA) gene, 29 tRNA genes and two nonidentical ribosomal RNA operons. One rRNA operon has a tRNAAla(UGC) gene between therrsand therrlgenes, whereas another has a tRNAIle(GAU) gene. Phylogenetic analyses suggest that the plastids of Heterokontophyta, Cryptophyta and Haptophyta originated from the subphylum Rhodophytina. The order of the genes in the ribosomal protein cluster of theP. purpureumplastid genome differs from that of other Rhodophyta and Chromalveolata. These results suggest that a large-scale rearrangement occurred in the plastid genome ofP. purpureumafter its separation from other Rhodophyta.