Electrophysiological study of parvocellular red nucleus neurons
Electrophysiological study of parvocellular red nucleus neurons
复制标题
小细胞红核神经元的电生理研究
DOI：
10.1016/0006-8993(78)90605-4
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发表时间：
1978
期刊：
影响因子：
2.9
通讯作者：
K. Jinnai
中科院分区：
文献类型：
--
作者：
H. Oka;K. Jinnai
一般认为，红核（RN）可大致分为尾侧大细胞部分和吻侧小细胞部分，但猫的这两个区域之间的边界并不清楚14，16。大部分的生理学和解剖学研究的RN已经进行了大细胞的一部分，他们已经阐明了红核传入和传出的组织，如代表的corticorubralla，15，17，23，interpositorubra 12，5，24，红核脊髓连接15，1 s，23。然而，RN的小细胞部分的研究一直到目前为止很少，并提出了很少的知识，这部分的功能意义。本实验在15只猫戊巴比妥钠浅麻醉状态下，用微电极细胞外观察了猫RN吻外侧部神经元，经对大脑皮质、小脑核和下橄榄核刺激的反应性和组织学定位，鉴定为小细胞RN神经元。开颅术暴露大脑半球和小脑。吸出枕叶皮层和海马后，显露出上级丘。通过抽吸切割小脑下脚，这可以通过略微抬起小脑的尾侧边缘来实现。将几对双极刺激电极放置在乙状前回（额叶运动皮层）的外侧和内侧部分以及中上回（顶叶联合皮层）的前部。根据Hassler和Muhs-Clement 10，大脑皮层的这些部分分别属于区域4、区域6和区域5。将四个刺激电极背侧引入大脑刺激侧的下橄榄，以识别红橄榄神经元。在这种情况下，通过在相邻的两个电极之间依次施加短脉冲电流来给予刺激。将三个同心刺激电极插入大脑刺激侧的对侧小脑核。记录玻璃微电极，角度约85 Horsley-Clarke平面，从暴露表面的上级丘推进到RN。以这种方式，RN可以通过微电极在距离丘表面7和9 mm之间的范围内到达。通过刺激电极向大脑皮层、小脑核或下橄榄传递单脉冲电流（持续时间为0.1- 0.2毫秒
As generally accepted, the red nucleus (RN) may be roughly divided into a caudal magnocellular part and a rostral parvocellular part but the boundary between the two regions is not clear in the cat14, 16. Most of physiological as well as anatomical studies of the RN have been performed on the magnocellular part and they have elucidated rubral afferent and efferent organizations as represented by the corticorubralla, 15, 17, 23, interpositorubra12, 5, 24, rubrospinal connectionslS, 1s, 23. However, studies of the parvocellular part of RN have been so far few and presented little knowledge on the functional significance of this part. In the present study, the neurons in the rostrolateral part of RN of the cat were explored with microelectrodes extracellularly and they were identified as the parvocellular RN neurons, both with their responsiveness to stimulation of the cerebral cortex, the cerebellar nucleus and the inferior olive, and with their localization in the histology.The experiments were performed on 15 cats under light anesthesia with sodium pentobarbital. Craniotomy was made to expose the cerebral hemisphere and the cerebellum. The superior colliculus was disclosed after sucking out the overlying occipital cortex and the hippocampus. The inferior cerebellar peduncle was cut by suction, which was possible by lifting up slightly the caudal edge of the cerebellum. Several pairs of bipolar stimulating electrodes were placed on the lateral and medial parts of the anterior sigmoid gyrus (frontal motor cortex) and the anterior part of the middle suprasylvian gyrus (parietal association cortex). These parts of the cerebral cortex belong respectively to area 4, area 6 and area 5 according to Hassler and Muhs-Clement 10. Four stimulating electrodes were introduced dorsally into the inferior olive ipsilateral to the stimulated side of the cerebrum, in order to identify the rubro-olivary neurons. In this case, stimulation was given by applying brief pulse current in turn between the neighboring two electrodes. Three concentric stimulating electrodes were inserted into the cerebellar nuclei contralateral to the stimulated side of the cerebrum. Recording glass microelectrodes, angled about 85 to the Horsley-Clarke plane, were advanced from the exposed surface of the superior colliculus to the RN. In this way, the RN could be reached by microelectrodes in a range between 7 and 9 mm from the surface of the colliculus. Single pulse currents were delivered through the stimulating electrodes to the cerebral cortex, the cerebellar nuclei or the inferior olive (0.1--0.2 msec in duration