Transcriptional repression of a hormone-responsive promoter.
Transcriptional repression of a hormone-responsive promoter.
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DOI：
10.1093/nar/15.17.6973
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发表时间：
1987-09
影响因子：
14.9
通讯作者：
Kimberlin L. Morley;Mark G. Toohey;D. O. Peterson
中科院分区：
文献类型：
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作者：
Kimberlin L. Morley;Mark G. Toohey;D. O. Peterson
小鼠乳腺肿瘤病毒启动子的活性在各种序列背景下通过瞬时转染试验进行评估，其中启动子活性是通过编码氯霉素乙酰转移酶的连锁基因的表达以及RNA转录物的直接分析来确定的。结果表明，除了先前描述的糖皮质激素应答转录增强子外，前病毒长末端重复序列还包含负转录控制元件。负元件能够在两个方向上起作用，并且至少在某种程度上，相对于受调节的转录单元在多个位置上起作用。对基因表达的影响不能用转染效率的改变来解释。该元件已定位于一个91碱基对片段，该片段位于糖皮质激素受体蛋白的5'结合位点上，已在体外确定。负性元件的作用可能是在缺乏糖皮质激素的情况下抑制原病毒启动子的固有活性，导致在存在和缺乏激素的情况下基因表达比例增加。
The activity of the mouse mammary tumor virus promoter was assessed in various sequence contexts with a transient transfection assay in which promoter activity was determined by way of expression of a linked gene encoding chloramphenicol acetyltransferase, as well as by direct analysis of RNA transcripts. The results indicate that the proviral long terminal repeat contains a negative transcriptional control element in addition to the glucocorticoid-responsive transcriptional enhancer that has been described previously. The negative element is able to function in both orientations and, at least to some extent, at multiple positions with respect to the regulated transcription unit. The effects on gene expression cannot be explained by alterations in transfection efficiency. The element has been localized to a 91 base pair fragment located immediately 5' of binding sites for the glucocorticoid receptor protein that have been defined in vitro. The role of the negative element may be to repress the inherent activity of the proviral promoter in the absence of glucocorticoids, resulting in an increased ratio of gene expression in the presence and absence of hormone.