Genistein-stimulated adherence of prostate cancer cells is associated with the binding of focal adhesion kinase to beta-1-integrin
Genistein-stimulated adherence of prostate cancer cells is associated with the binding of focal adhesion kinase to beta-1-integrin
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DOI：
10.1007/bf00123398
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发表时间：
1996-09-01
影响因子：
4
通讯作者：
Neckers, L
中科院分区：
文献类型：
--
作者：
Bergan, R;Kyle, E;Neckers, L
金雀异黄素是一种蛋白酪氨酸激酶抑制剂，被认为是一种潜在的癌症预防药物，它的消耗与前列腺癌的临床低转移率有关面对器官受限前列腺癌的持续高发。因此，我们开展了金雀异黄素对Tell黏附的影响的研究，作为其可能的抗转移机制之一：形态分析发现金雀异黄素引起多种细胞系：PC3-M，PC3，DU-145前列腺癌和MCF-7乳腺癌细胞的细胞扁平化，机制研究主要集中在高转移的PC3-M细胞系，PC3-M细胞系，DU-145细胞系和MCF-7乳腺癌细胞系。并发现细胞扁平化伴随着细胞黏附增加，进一步的研究表明粘着斑激酶(FAK)在局部细胞黏附区域积聚，这种积聚仅在细胞积极经历染料木素介导的形态变化时发生，细胞黏附分子、β-1-整合素和FAK之间同时形成复合体，并与FAK活性的瞬时激活相关，Genistein被认为是一种新的研究工具，用于研究参与细胞黏附过程的分子事件。
The isoflavinoid genistein is a protein-tyrosine kinase inhibitor which has been identified as a putative cancer prevention agent, Its consumption is associated with a low incidence of clinical metastatic prostate cancer in the face of a sustained high incidence of organ-confined prostate cancer, We therefore undertook studies to examine genistein's effect upon tell adhesion as one possible mechanism by which it could be acting as an antimetastatic: agent, A morphogenic analysis revealed that genistein caused cell flattening in a variety of cell lines: PC3-M, PC3, and DU-145 prostate carcinoma cells, as well as MCF-7 breast carcinoma cells, Mechanistic studies focused on the highly metastatic PC3-M cell Line, and revealed that cell flattening was accompanied by an increase in cell adhesion, Further investigations demonstrated that focal adhesion kinase (FAK) accumulated in areas of focal cell attachment, and that this accumulation occurred only when cells were actively undergoing genistein-mediated morphologic change, Concurrent formation of a complex between the cell attachment molecule, beta-1-integrin, and FAK was shown to occur, and to correlate with transient activation of FAK activity, Genistein is presented as a novel investigative tool for use in the study of molecular events involved in the process of cell adhesion.