Effects of Malignant Melanoma Initiating Cells on T-Cell Activation.
Effects of Malignant Melanoma Initiating Cells on T-Cell Activation.
复制标题
恶性黑色素瘤起始细胞对 T 细胞激活的影响。
DOI：
10.1007/7651_2015_299
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Frank,MarkusH
中科院分区：
文献类型：
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作者：
Schatton,Tobias;Schütte,Ute;Frank,MarkusH
虽然人类恶性黑色素瘤是一种高度免疫原性的癌症，但内源性抗肿瘤免疫应答和黑色素瘤免疫治疗往往无法控制肿瘤进展。因此，表征能够逃避抗肿瘤免疫的黑色素瘤细胞亚群可以揭示可能降低黑色素瘤发病率和死亡率的优化治疗策略。恶性黑色素瘤起始细胞（MMIC）具有调节抗肿瘤免疫应答和驱动肿瘤生长和进展的优先能力，因此需要对其进行更深入的研究，以进一步阐明黑色素瘤免疫逃避和免疫治疗抗性的细胞和分子机制。在这里，我们描述的方法，使纯化的MMIC与黑色素瘤批量人口的免疫调节作用的特性与同基因或同种异体淋巴细胞共培养，使用[3H]胸苷掺入，酶联免疫吸附斑点（ELISPOT），或ELISA检测。这些试验传统上被开发用于分析同种免疫过程，我们成功地将其用于肿瘤介导的免疫调节功能的研究。
Although human malignant melanoma is a highly immunogenic cancer, both the endogenous antitumor immune response and melanoma immunotherapy often fail to control neoplastic progression. Accordingly, characterizing melanoma cell subsets capable of evading antitumor immunity could unravel optimized treatment strategies that might reduce morbidity and mortality from melanoma. By virtue of their preferential capacity to modulate antitumor immune responses and drive inexorable tumor growth and progression, malignant melanoma-initiating cells (MMICs) warrant closer investigation to further elucidate the cellular and molecular mechanisms underlying melanoma immune evasion and immunotherapy resistance. Here we describe methodologies that enable the characterization of immunoregulatory effects of purified MMICs versus melanoma bulk populations in coculture with syngeneic or allogeneic lymphocytes, using [3H]thymidine incorporation, enzyme-linked immunosorbent spot (ELISPOT), or ELISA assays. These assays were traditionally developed to analyze alloimmune processes and we successfully adapted them for the study of tumor-mediated immunomodulatory functions.