Čerenkov excited fluorescence tomography using external beam radiation.
Čerenkov excited fluorescence tomography using external beam radiation.
复制标题
切连科夫利用外部光束辐射激发荧光断层扫描。
DOI：
10.1364/ol.38.001364
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发表时间：
2013-04-15
期刊：
影响因子：
3.6
通讯作者：
Pogue BW
中科院分区：
文献类型：
--
作者：
Demers JL;Davis SC;Zhang R;Gladstone DJ;Pogue BW
癌症的放射治疗在整个治疗体积中诱导光学Čerenkov发射，其可用于在体内激发分子报告物，从而允许在分次治疗期间对组织反应进行分子感测。在这封信中，这个信号的空间映射可以实现与荧光团分布的断层扫描恢复的想法进行了测试，第一次，使用6兆电子伏的光子从线性加速器在一个异质组织幻影。Čerenkov光激发的荧光团在整个组织模型，和扩散断层扫描被用来恢复图像。从13个位置的测量，光谱仪检测和光谱拟合，分离的荧光团发射的Čerenkov连续。荧光扩散断层图像显示浓度和重建值之间的线性响应。将这种分子成像应用于分子报告治疗的潜力似乎很有希望。
Radiation treatment of cancer induces an optical Čerenkov emission throughout the treated volume, which could be used to excite molecular reporters in vivo, allowing molecular sensing of tissue response during fractionated therapy. In this letter, the idea that spatial mapping of this signal can be achieved with tomographic recovery of the fluorophore distribution is tested for the first time, using 6 MeV photons from a linear accelerator in a heterogeneous tissue phantom. Čerenkov light excited fluorophore throughout the tissue phantom, and diffuse tomography was used to recover images. Measurements from13 locations were used, with spectrometer detection and spectral fitting, to separate the fluorophore emission from the Čerenkov continuum. Fluorescent diffuse tomographic images showed a linear response between the concentration and the reconstructed values. The potential to apply this molecular imaging in treatment with molecular reporters appears promising.