Direct PCR detection of Escherichia coli O157:H7
Direct PCR detection of Escherichia coli O157:H7
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DOI：
10.1046/j.1472-765x.2003.01386.x
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发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
Collins, MLP
中科院分区：
文献类型：
--
作者：
Fode-Vaughan, KA;Maki, JS;Collins, MLP
目的：报道一种简便、快速的检测产志贺毒素(STX)大肠杆菌(STEC)的方法。方法和结果：直接聚合酶链式反应(DPCR)不需要在聚合酶链式反应前从样品中回收细胞或提取DNA。利用STX编码基因stx1和stx2的特异性引物对环境水样和牛奶中的O157：H7大肠杆菌进行dPCR检测。结论：只含一个细胞的聚合酶链式反应产生了一种dPCR产物。
Aims: This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC).Methods and Results: Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk.Conclusions: PCR reactions containing one cell yielded a DPCR product.Significance and Impact of the Study: This should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.