SPECIFIC CLEAVAGE OF MODEL RECOMBINATION AND REPAIR INTERMEDIATES BY THE YEAST RAD1-RAD10 DNA ENDONUCLEASE
SPECIFIC CLEAVAGE OF MODEL RECOMBINATION AND REPAIR INTERMEDIATES BY THE YEAST RAD1-RAD10 DNA ENDONUCLEASE
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DOI：
10.1126/science.8091230
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发表时间：
1994-09-30
期刊：
影响因子：
56.9
通讯作者：
FRIEDBERG, EC
中科院分区：
文献类型：
--
作者：
BARDWELL, AJ;BARDWELL, L;FRIEDBERG, EC
酿酒酵母的RAD 1和RAD 10基因是核苷酸切除修复和某些有丝分裂重组事件所必需的。在这里，模型重组和修复中间体被用来表明，Rad 1-Rad 10介导的切割发生在双链单链连接。此外，切割仅发生在含有3'单链尾的链上。因此，生物化学和遗传学的证据表明，在特定的重组中间体的切割的Rad 1-Rad 10复合物的作用。此外，这些数据表明，Rad 1-Rad 10核酸内切酶在核苷酸切除修复期间将DNA 5'切割至受损碱基。
The RAD1 and RAD10 genes of Saccharomyces cerevisiae are required for both nucleotide excision repair and certain mitotic recombination events. Here, model recombination and repair intermediates were used to show that Rad1-Rad10-mediated cleavage occurs at duplex-single-strand junctions. Moreover, cleavage occurs only on the strand containing the 3' single-stranded tail. Thus, both biochemical and genetic evidence indicate a role for the Rad1-Rad10 complex in the cleavage of specific recombination intermediates. Furthermore, these data suggest that Rad1-Rad10 endonuclease incises DNA 5' to damaged bases during nucleotide excision repair.