Glucose transporter-1 expression in renal cell carcinoma and its correlation with hypoxia inducible factor-1α
Glucose transporter-1 expression in renal cell carcinoma and its correlation with hypoxia inducible factor-1α
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DOI：
10.1111/j.1464-410x.2007.07238.x
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发表时间：
2008-02-01
影响因子：
4.5
通讯作者：
Ljungberg, Borje
中科院分区：
文献类型：
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作者：
Lidgren, Anders;Bergh, Anders;Ljungberg, Borje
缺氧诱导因子-1 α转录因子通过使用组织微阵列（TMA）分析不同类型肾细胞癌中HIF-1 α的靶基因葡萄糖转运蛋白-1（GLUT-1），（RCC，具有可变临床过程的肿瘤，部分由于血管生成活性），因为血管生成对于肿瘤进展和转移扩散是重要的，并被缺氧激活。使用制备的TMA的免疫组织化学染色，对187例患者的样本进行了半定量分析，其中包括148例传统型、26例乳头状和13例嫌色细胞癌。GLUT-1染色主要存在于细胞质中。根据染色强度将肿瘤细分为GLUT - 1（低）和GLUT-1（高）。GLUT-1在肾癌组织中的表达在不同类型间差异有统计学意义（P < 0.05）。在常规肾癌中，GLUT-1与临床病理变量无关。相比之下，乳头状肾细胞癌与肿瘤分期相关。在常规和乳头状肾细胞癌中，GLUT-1（LOW）表达的患者生存率更高的趋势不明显。GLUT-1与HIF-1 α表达显著相关（P=0.008），常规肾细胞癌患者GLUT-1（高）表达较多，与HIF-1 α表达显著相关。在乳头状肾细胞癌中，GLUT-1表达与分期相关; GLUT-1表达在常规肾细胞癌中显著高于乳头状和嫌色肾细胞癌。GLUT-1（低）在乳头状和常规RCC似乎对应于较好的预后。
To evaluate transcription factor hypoxia inducible factor-1 alpha (HIF-1 alpha) activity, by analysing a target gene for HIF-1 alpha, glucose transporter-1 (GLUT-1), using a tissue microarray (TMA) in different types of renal cell carcinoma (RCC, a tumour with a variable clinical course, partly due to angiogenic activity), as angiogenesis is important for tumour progression and metastatic spread, and is activated by hypoxia.GLUT-1 and HIF-1 alpha expressions were semiquantitatively analysed using immunohistological staining of a prepared TMA, using samples from 187 patients, including 148 with conventional, 26 with papillary and 13 with chromophobe RCC.GLUT-1 staining was found mainly in the cytoplasm. The tumours were subdivided into GLUT - 1(LOW) and GLUT-1(HIGH), based on staining intensity. There was a significant difference in GLUT-1 expression between RCC types (P < 0.05). In conventional RCC, GLUT-1 had no correlation with clinicopathological variables. By contrast there was a correlation with tumour stage in papillary RCC. There was an insignificant trend to better survival of patients with GLUT-1(LOW) expression in both conventional and papillary RCC. GLUT-1 correlated significantly (P=0.008) with HIF-1 alpha.Most patients with conventional RCC had GLUT-1(HIGH) staining and there was a significant correlation with HIF-1 alpha. In papillary RCC, GLUT-1 expression was associated with stage; GLUT-1 expression was significantly higher in conventional RCC than in papillary and chromophobe RCC. GLUT-1(LOW) in both papillary and conventional RCC appeared to correspond with a better prognosis.