Triphenyltin chloride induces spindle microtubule depolymerisation and inhibits meiotic maturation in mouse oocytes.
Triphenyltin chloride induces spindle microtubule depolymerisation and inhibits meiotic maturation in mouse oocytes.
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DOI：
10.1071/rd12332
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发表时间：
2014-10
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通讯作者：
Yu-ting Shen;Yue-qiang Song;Xiao-qin He;Fei Zhang;Xin Huang;Yu Liu;L. Ding;Lin Xu;Mao-Bi Zhu;Wen Hu;Zhong-quan Qi;Hai-Long Wang;Xiang-Jun Yang
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作者：
Yu-ting Shen;Yue-qiang Song;Xiao-qin He;Fei Zhang;Xin Huang;Yu Liu;L. Ding;Lin Xu;Mao-Bi Zhu;Wen Hu;Zhong-quan Qi;Hai-Long Wang;Xiang-Jun Yang
减数分裂产生单倍体配子进行有性生殖。三苯基氯化锡（TPTCL）是一种高度生物积累和有毒的环境雌激素；然而，其对卵母细胞减数分裂的影响尚不清楚。在体外和体内研究了TPTCL对小鼠卵母细胞减数分裂成熟的影响。在体外，TPTCL以剂量依赖的方式抑制生发囊泡破裂（GVBD）和第一极体挤压（PBE）。卵母细胞暴露于5或10μgmL(-1) TPTCL后，纺锤体微管完全解体，染色体凝聚。γ-微管蛋白异常地定位在染色体附近而不是纺锤杆上。在体内，小鼠灌胃TPTCL治疗10天。小鼠总体状况恶化，卵巢系数降低（P0.05）。10mgkg(-1) TPTCL抑制PBE （P<0.05）。用10mgkg(-1) TPTCL拆解体内和体外中期卵母细胞的纺锤体。这些结果表明，TPTCL通过干扰细胞周期进程、干扰微管细胞骨架和抑制卵泡发育严重影响小鼠卵母细胞的减数分裂成熟。
Meiosis produces haploid gametes for sexual reproduction. Triphenyltin chloride (TPTCL) is a highly bioaccumulated and toxic environmental oestrogen; however, its effect on oocyte meiosis remains unknown. We examined the effect of TPTCL on mouse oocyte meiotic maturation in vitro and in vivo. In vitro, TPTCL inhibited germinal vesicle breakdown (GVBD) and first polar body extrusion (PBE) in a dose-dependent manner. The spindle microtubules completely disassembled and the chromosomes condensed after oocytes were exposed to 5 or 10μgmL(-1) TPTCL. γ-Tubulin protein was abnormally localised near chromosomes rather than on the spindle poles. In vivo, mice received TPTCL by oral gavage for 10 days. The general condition of the mice deteriorated and the ovary coefficient was reduced (P0.05). PBE was inhibited with 10mgkg(-1) TPTCL (P<0.05). The spindles of in vitro and in vivo metaphase II oocytes were disassembled with 10mgkg(-1) TPTCL. These results suggest that TPTCL seriously affects meiotic maturation by disturbing cell-cycle progression, disturbing the microtubule cytoskeleton and inhibiting follicle development in mouse oocytes.