Reporter gene technology to assess activity of antimycobacterial agents in macrophages
Reporter gene technology to assess activity of antimycobacterial agents in macrophages
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DOI：
10.1128/aac.40.6.1542
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发表时间：
1996-06-01
影响因子：
4.9
通讯作者：
Stover, CK
中科院分区：
文献类型：
--
作者：
Arain, TM;Resconi, AE;Stover, CK
利用内源性表达萤火虫荧光素酶的结核分枝杆菌和牛分枝杆菌卡介苗报告菌株进行生物发光试验，评价异烟肼和利福平对人巨噬细胞中分离的分枝杆菌的活性。该方法允许评估抗生素对细胞内分枝杆菌的疗效，而无需进行与传统CFU测定相关的劳动密集型程序和长时间孵育要求。
Reporter strains of Mycobacterium tuberculosis and Mycobacterium bovis BCG endogenously expressing firefly luciferase were used in bioluminescence assays to evaluate the activities of isoniazid and rifampin against mycobacteria sequestered in human macrophages. This methodology allowed the efficacy of antibiotics against intracellular mycobacteria to be assessed without the labor-intensive procedures and protracted incubation requirements associated with conventional CFU determinations.