Differential Distribution and Energy Status-Dependent Regulation of the Four CART Neuropeptide Genes in the Zebrafish Brain
Differential Distribution and Energy Status-Dependent Regulation of the Four CART Neuropeptide Genes in the Zebrafish Brain
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DOI：
10.1002/cne.23532
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发表时间：
2014-07-01
影响因子：
2.5
通讯作者：
Ghose, Aurnab
中科院分区：
文献类型：
--
作者：
Akash, G.;Kaniganti, Tarun;Ghose, Aurnab
可卡因和安非他明调节的转录物（CART）神经肽已被牵连在整个脊椎动物门的能量稳态的神经调节。通过基因特异性原位杂交，我们绘制了四个CART mRNA在成年斑马鱼中枢神经系统中的分布。CART 2和4的广泛神经元表达模式表明，该肽在处理来自包括嗅觉和视觉输入在内的各种方式的感觉信息中具有突出作用。相比之下，CART 1和3的分布更为有限，主要分别位于内侧纵束核（NMLF）和脚内核（EN）。CART 2和4在视前区和结节区的富集强调了CART在神经内分泌功能中的重要性。饥饿导致CART阳性细胞在外侧隐窝核（NRL）和结节外侧核（NLT）下丘脑区域的显着减少，这表明这些神经元的能量稳态功能。同样，EN作为一种新的能量状态响应区域出现。不仅有丰富的和重叠的CART 2，3和4的表达在EN，而且饥饿诱导减少CART表达神经元在这个区域。CART mRNA的细胞分辨率映射和CART表达核对饥饿的反应强调了CART神经肽在能量加工中的重要性。此外，区域和基因特异性反应的能量水平表明一个复杂的，互动的网络，其中四个CART基因产物可能有非冗余功能的能量稳态。J. Comp.神经元522：2266-2285，2014。(c)2013 Wiley Periodicals，Inc.
The cocaine- and amphetamine-regulated transcript (CART) neuropeptide has been implicated in the neural regulation of energy homeostasis across vertebrate phyla. By using gene-specific in situ hybridization, we have mapped the distribution of the four CART mRNAs in the central nervous system of the adult zebrafish. The widespread neuronal expression pattern for CART 2 and 4 suggests a prominent role for the peptide in processing sensory information from diverse modalities including olfactory and visual inputs. In contrast, CART 1 and 3 have a much more restricted distribution, predominantly located in the nucleus of the medial longitudinal fasciculus (NMLF) and entopeduncular nucleus (EN), respectively. Enrichment of CART 2 and 4 in the preoptic and tuberal areas emphasizes the importance of CART in neuroendocrine functions. Starvation resulted in a significant decrease in CART-positive cells in the nucleus recessus lateralis (NRL) and nucleus lateralis tuberis (NLT) hypothalamic regions, suggesting a function in energy homeostasis for these neurons. Similarly, the EN emerges as a novel energy status-responsive region. Not only is there abundant and overlapping expression of CART 2, 3, and 4 in the EN, but also starvation induced a decrease in CART-expressing neurons in this region. The cellular resolution mapping of CART mRNA and the response of CART-expressing nuclei to starvation underscores the importance of CART neuropeptide in energy processing. Additionally, the regional and gene-specific responses to energy levels suggest a complex, interactive network whereby the four CART gene products may have nonredundant functions in energy homeostasis. J. Comp. Neurol. 522:2266-2285, 2014. (c) 2013 Wiley Periodicals, Inc.