Cytotoxicity and related effects of T-2 toxin on cultured Vero cells
Cytotoxicity and related effects of T-2 toxin on cultured Vero cells
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DOI：
10.1016/j.toxicon.2006.06.004
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发表时间：
2006-09-01
期刊：
影响因子：
2.8
通讯作者：
Bacha, H.
中科院分区：
文献类型：
--
作者：
Bouaziz, C.;Abid-Essefi, S.;Bacha, H.
T-2毒素是由镰刀菌合成的一类真菌毒素，广泛作为某些重要农产品特别是谷物的天然污染物存在。一旦接触，T-2毒素会引起严重的人类和动物疾病。它被认为是人类致命的消化道毒性阿柳中毒（ATA）的主要病原体。本研究采用MTT法和中性红活力法研究T-2毒素对体外Vero细胞株的细胞毒性和诱导凋亡，诱导脂质过氧化，降低大分子（蛋白质、DNA和RNA）水平，DNA断裂，诱导caspase-3依赖性凋亡。我们的研究结果表明，T-2毒素降低细胞活力与大分子水平的损害相关。琼脂糖凝胶电泳显示DNA阶梯状，诱导DNA断裂。这种断裂与凋亡诱导有关，这一点通过caspase-3的激活和反映线粒体功能障碍的线粒体膜电位的耗尽得到证实。(c) 2006 Elsevier Ltd.版权所有。
T-2 toxin belongs to a group of mycotoxins synthesized by Fusarium fungi that are widely encountered as natural contaminants of certain important agricultural commodities particularly, cereals. Upon exposure, T-2 toxin causes severe human and animal diseases. It is considered to be a major causative agent in fatal alimentary toxic aleukia (ATA) in humans. In this study, cytotoxicity and apotosis induction by T-2 toxin was investigated in vitro on Vero cell line using the MTT and the neutral red viability assays, the induction of lipid peroxidation, the decrease of macromolecule levels (protein, DNA and RNA), DNA fragmentation and caspase-3-dependent apoptosis induction. Our results showed that T-2 toxin reduced cell viability correlated to an impairment of macromolecule levels. It also increased MDA formation, induced DNA fragmentation showed by DNA laddering patterns on agarose gel electrophoresis. This fragmentation is in relation with apoptosis induction which was confirmed by activation of caspase-3, and depletion of the mitochondrial membrane potential reflecting a mitochondrial dysfunction. (c) 2006 Elsevier Ltd. All rights reserved.