A rat model of bone inflammation-induced pain by intra-tibial complete Freund's adjuvant injection
A rat model of bone inflammation-induced pain by intra-tibial complete Freund's adjuvant injection
复制标题
DOI：
10.1016/j.neulet.2010.12.027
复制
发表时间：
2011-03-03
影响因子：
2.5
通讯作者：
Mao-Ying, Qi-Liang
中科院分区：
文献类型：
--
作者：
Yang, Chang-Jiang;Wang, Xiao-Wei;Mao-Ying, Qi-Liang
在先前的研究中，通过将完全弗氏佐剂（CFA）或辣椒素直接注射到深部躯体组织或动物的后爪中来产生炎性疼痛模型。相反，通过将肿瘤细胞注射到股骨或胫骨的空腔中来模拟骨癌引起的疼痛（BCIP）。据报道，由于传入神经支配的差异，对不同组织类型的相同刺激可能导致不同的疼痛反应模式。因此，本研究的目的是通过将CFA注射到BCIP模型中涉及的相同部位的胫骨腔中来建立骨炎症诱导疼痛（BIIP）的大鼠模型。评价体重、骨组织学、机械异常性疼痛、热痛觉过敏以及西乐葆对该BHP模型的镇痛作用的差异。结果显示，在胫骨内CFA注射后两天，在骨髓中观察到显著炎症的证据，包括核凝聚和碎裂、大量嗜中性粒细胞和显著的嗜中性渗出物。注射后14天，通过组织学染色检测到骨的显著纤维化。在单侧CFA注射后，行为学研究显示对von Frey毛发刺激的机械异常性疼痛，但没有观察到热痛觉过敏。西乐葆对BHP模型显示出显著的抗异常性疼痛作用。实验结果表明，CFA可有效诱导大鼠骨炎症反应及疼痛相关行为，为BCIP的研究提供了一个有价值的实验对照。(C)2010爱思唯尔爱尔兰有限公司版权所有。
In prior studies, models of inflammatory pain were produced through injecting complete Freund's adjuvant (CFA) or capsaicin directly into either the deep somatic tissue or the animal's hind paw. In contrast, bone cancer-induced pain (BCIP) was simulated through injecting tumor cells into the cavity of the femur or the tibia. It has been reported that, due to differences in afferent innervation, the same stimulus to various tissue types might result in differing patterns of pain response. Hence, the aim of this study is to establish a rat model of bone inflammation-induced pain (BIIP) by injecting CFA into the tibial cavity, the same site involved in the BCIP model. The differences in body weight, bone histology, mechanical allodynia, thermal hyperalgesia, and the pain relieving effects of Celebrex on this model of BHP were evaluated. The results showed that there was evidence of significant inflammation seen in the bone marrow two days after intra-tibial CFA injection, including nuclear condensation and fragmentation, massive neutrophilic granulocytes, and prominent fibrinous exudates. Fourteen days after injection, marked fibrosis of the bone was detected by histological staining. After unilateral CFA injection, behavioral studies showed mechanical allodynia to von Frey hair stimulation, but no thermal hyperalgesia was observed. Celebrex showed significant anti-allodynic effects on the BHP model. The results demonstrated that CFA is an effective agent for inducing bone inflammation and subsequent pain-related behavior in rat models, and, thus, provides a practical and valuable contrast for BCIP research. (C) 2010 Elsevier Ireland Ltd. All rights reserved.