Potassium channels regulate hypo-osmotic shock-induced motility of common carp (Cyprinus carpio) sperm
Potassium channels regulate hypo-osmotic shock-induced motility of common carp (Cyprinus carpio) sperm
复制标题
钾通道调节低渗休克引起的鲤鱼精子运动
DOI：
10.1016/0044-8486(94)00234-f
复制
发表时间：
1995
期刊：
影响因子：
4.5
通讯作者：
L. Trón
中科院分区：
文献类型：
--
作者：
Z. Krasznai;T. Márián;L. Balkay;R. Gáspár;L. Trón
鱼精浆的渗透压和成分通常会抑制精子在睾丸和精子管中的运动。在产卵时环境渗透压的变化激活了精子尾巴的鞭毛运动。通过显微运动试验研究了阴离子和阳离子通道抑制剂对主动运动的影响。钠通道抑制剂（阿米洛利和河豚毒素）和阴离子通道抑制剂（DIDS和乙酸）不影响鞭毛运动，而钾通道抑制剂（4-AP、奎宁、缬草碱、维拉帕米）减少鞭毛运动的持续时间或完全消除鞭毛运动。钾通道抑制剂对精子活力的影响取决于孵育的剂量和时间。电压激活的K+阻滞剂4-氨基吡啶被证明是最有效的抑制剂。其作用是完全可逆的，表明钾离子通道的功能状态对精子的激活机制至关重要。用荧光流式细胞术测定精子胞内pH值。低渗透休克诱导鲤鱼精子细胞内碱化。pH值增加0.15 pH单位。在4-AP存在的情况下，pH变化的测量振幅和动力学与没有这种抑制剂的情况相同，这表明细胞内pH变化的调节作用是不存在的。
The osmolality and composition of fish seminal plasma usually suppress sperm motility in the testis and sperm duct. Change in the osmolality of the environment at spawning activates flagellar motion of the sperm tail. The effect of inhibitors of anion and cation channels on active motion was investigated by a microscopic motility test. While sodium channel inhibitors (amiloride and tetrodotoxin) as well as anion channel inhibitors (DIDS and ethacrynic acid) did not affect motility, potassium channel inhibitors (4-AP, quinine, veratrine, verapamil) decreased the duration of flagellar motion or abolished motility completely. Potassium channel inhibitors exerted an effect on sperm motility depending on the dose and length of incubation. The voltage-activated K+blocker 4-aminopiridine proved to be the most potent inhibitor. Its effect was completely reversible, indicating that the functional state of potassium channels is essential to the activation mechanism of sperm. Intracellular pH of the spermatozoa was measured by a fluorescent flow cytometric technique. Hypo-osmotic shock induced intracellular alkalinization of carp sperm. Increase in pHiwas 0.15 pH units. In the presence of 4-AP the measured amplitude and kinetics of the pHichange was the same as in the absence of this inhibitor, arguing against a regulatory role of intracellular pH change.