Grape Seed and Skin Extract Prevents High-Fat Diet-Induced Brain Lipotoxicity in Rat
Grape Seed and Skin Extract Prevents High-Fat Diet-Induced Brain Lipotoxicity in Rat
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DOI：
10.1007/s11064-012-0821-2
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发表时间：
2012-09-01
影响因子：
4.4
通讯作者：
Aouani, Ezzedine
中科院分区：
文献类型：
--
作者：
Charradi, Kamel;Elkahoui, Salem;Aouani, Ezzedine
肥胖与患痴呆症的风险增加有关，而脂肪对大脑产生不利影响的生理机制尚不清楚。本研究分析了高脂饮食(HFD)对大鼠脑脂肪变性和氧化应激的影响，以及参与信号转导的细胞内介质，以及葡萄籽和葡萄皮提取物(GSSE)的保护作用。HFD诱导胆固醇和磷脂的异位沉积，但不诱导甘油三酯的异位沉积。此外，脑脂毒性与氧化应激有关，其特征是脂质过氧化和羰化增加，谷胱甘肽过氧化物酶和超氧化物歧化酶活性抑制，锰的枯竭，以及伴随而来的可电离钙和乙酰胆碱酯酶活性的增加。重要的是，GSSE减轻了HFD治疗的所有有害影响。综上所述，HFD在治疗锰中毒方面有一定的应用前景，GSSE可作为一种安全的抗脂毒药用于防治脂肪性脑损伤。
Obesity is related to an elevated risk of dementia and the physiologic mechanisms whereby fat adversely affects the brain are poorly understood. The present investigation analyzed the effect of a high fat diet (HFD) on brain steatosis and oxidative stress and the intracellular mediators involved in signal transduction, as well as the protection offered by grape seed and skin extract (GSSE). HFD induced ectopic deposition of cholesterol and phospholipid but not triglyceride. Moreover brain lipotoxicity is linked to an oxidative stress characterized by increased lipoperoxidation and carbonylation, inhibition of glutathione peroxidase and superoxide dismutase activities, depletion of manganese and a concomitant increase in ionizable calcium and acetylcholinesterase activity. Importantly GSSE alleviated all the deleterious effects of HFD treatment. Altogether our data indicated that HFD could find some potential application in the treatment of manganism and that GSSE should be used as a safe anti-lipotoxic agent in the prevention and treatment of fat-induced brain injury.