Hydrogen-rich water inhibits glucose and α,β -dicarbonyl compound-induced reactive oxygen species production in the SHR.Cg-Leprcp/NDmcr rat kidney.
Hydrogen-rich water inhibits glucose and α,β -dicarbonyl compound-induced reactive oxygen species production in the SHR.Cg-Leprcp/NDmcr rat kidney.
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DOI：
10.1186/2045-9912-2-18
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发表时间：
2012-07-09
影响因子：
2.9
通讯作者：
Shido O
中科院分区：
文献类型：
--
作者：
Katakura M;Hashimoto M;Tanabe Y;Shido O
α、β-二羰基化合物和晚期糖基化终产物诱导的活性氧（ROS）产生可导致2型糖尿病和代谢综合征患者肾功能障碍。富氢水（HRW）增加血液和组织中的H2水平，从而减少动物和人类的氧化应激。在本研究中，我们在体外和体内研究了HRW对葡萄糖和α，β-二羰基化合物诱导的ROS生成的影响。Wistar大鼠肾脏匀浆与葡萄糖和含HRW的α，β-二羰基化合物体外孵育，随后测定ROS水平。代谢综合征（SHR）体内动物模型。用HRW治疗Cg-Leprcp/NDmcr大鼠16周后，用液相色谱-质谱法测定肾脏ROS生成和血浆及肾脏α，β-二羰基化合物水平。HRW可抑制葡萄糖和α，β-二羰基化合物诱导的Wistar大鼠肾均质液中ROS的产生。此外,月。HRW处理的Cg-Leprcp/NDmcr大鼠ROS生成减少34%。此外，肾脏乙二醛、甲基乙二醛和3-脱氧葡萄糖酮水平分别下降81%、77%和60%。肾脏ROS水平与肾乙二醛（r = 0.659, p = 0.008）和甲乙二醛（r = 0.782, p = 0.001）水平呈正相关。这些结果表明，HRW抑制了SHR肾脏中α，β-二羰基化合物和ROS的产生。Cg-Leprcp / NDmcr老鼠。因此，它具有治疗2型糖尿病和代谢综合征患者肾功能障碍的潜力。
Reactive oxygen species (ROS) production induced by α,β-dicarbonyl compounds and advanced glycation end products causes renal dysfunction in patients with type 2 diabetes and metabolic syndrome. Hydrogen-rich water (HRW) increases the H2 level in blood and tissues, thus reducing oxidative stress in animals as well as humans. In this study, we investigated the effects of HRW on glucose- and α,β-dicarbonyl compound-induced ROS generation in vitro and in vivo. Kidney homogenates from Wistar rats were incubated in vitro with glucose and α,β-dicarbonyl compounds containing HRW, following which ROS levels were measured. In vivo animal models of metabolic syndrome, SHR.Cg-Leprcp/NDmcr rats, were treated with HRW for 16 weeks, following which renal ROS production and plasma and renal α,β-dicarbonyl compound levels were measured by liquid chromatograph mass spectrometer. HRW inhibited glucose- and α,β-dicarbonyl compound-induced ROS production in kidney homogenates from Wistar rats in vitro. Furthermore, SHR.Cg-Leprcp/NDmcr rats treated with HRW showed a 34% decrease in ROS production. Moreover, their renal glyoxal, methylglyoxal, and 3-deoxyglucosone levels decreased by 81%, 77%, and 60%, respectively. Positive correlations were found between renal ROS levels and renal glyoxal (r = 0.659, p = 0.008) and methylglyoxal (r = 0.782, p = 0.001) levels. These results indicate that HRW inhibits the production of α,β-dicarbonyl compounds and ROS in the kidneys of SHR.Cg-Leprcp/NDmcr rats. Therefore, it has therapeutic potential for renal dysfunction in patient with type 2 diabetes and metabolic syndrome.