MEASUREMENT OF DNA CONTENT AND CELL-VOLUME IN SENESCENT HUMAN FIBROBLASTS UTILIZING FLOW MULTIPARAMETER SINGLE CELL ANALYSIS
MEASUREMENT OF DNA CONTENT AND CELL-VOLUME IN SENESCENT HUMAN FIBROBLASTS UTILIZING FLOW MULTIPARAMETER SINGLE CELL ANALYSIS
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DOI：
10.1016/0014-4827(76)90441-9
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发表时间：
1976-01-01
影响因子：
3.7
通讯作者：
FOWLKES, BJ
中科院分区：
文献类型：
--
作者：
SCHNEIDER, EL;FOWLKES, BJ
用流式细胞荧光法分析衰老培养的人成纤维细胞的细胞周期，发现g1期细胞比例增加。在g1和g2 + M衰老细胞中，细胞DNA含量的变化增加，细胞DNA含量略有下降。利用门控单参数分析，在这些衰老成纤维细胞中观察到的细胞体积增加被证明存在于g1和G2 + M细胞中。
Cell cycle analysis of senescent cultured human fibroblasts by flow cytofluorometry reveals an increased proportion of cells in the G 1 period. An increased variation as well as a slight decrease in cellular DNA contents were observed in both G 1 and G 2 + M senescent cells. Utilizing gated single parameter analysis, the increased cell volumes observed in these senescent fibroblasts were demonstrated to be present in G 1 as well as G2 + M cells.