Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis
Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis
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PERK-eIF2α-CHOP信号通路在针刀治疗膝关节软骨细胞凋亡中的作用
DOI：
10.1155/2019/7164916
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发表时间：
2019-06-16
影响因子：
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通讯作者：
Huang, Xiao-shuang
中科院分区：
文献类型：
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作者：
Yang, Yong-hui;Liu, Tian-hao;Huang, Xiao-shuang
针刀疗法是针灸疗法的一种，已广泛应用于临床治疗膝骨关节炎（KOA）。然而，其机制尚不清楚。因此，我们以PERK-eIF2α-CHOP通路为起点，研究针刀干预兔KOA的作用机制，以期明确膝关节软骨细胞凋亡的机制。通过病理学研究和透射电镜检查关节软骨的细胞凋亡和超微结构变化，并分别通过qRT-PCR和蛋白质印迹检测PERK、eIF2α和CHOP mRNA和蛋白水平。模型组PERK、eIF2α、CHOP蛋白含量显着高于正常组（P < 0.01），针刀组和药物组较模型组显着下调（P < 0.01）。针刀组和药物组之间的eIF2α、p-eIF2α和CHOP蛋白水平没有显着差异。药物组PERK、eIF2α、CHOP mRNA水平均高于针刀组（P < 0.01）。针刀治疗可调节PERK-eIF2α-CHOP信号通路，这可能是其影响KOA兔软骨细胞凋亡的机制之一。
Needle knife therapy, a form of acupuncture and moxibustion, has been widely used in the clinical treatment of knee osteoarthritis (KOA). However, the mechanism is not clear. Therefore, we studied the mechanisms of action of needle knife intervention on KOA in rabbits, with the PERK-eIF2α-CHOP pathway as a starting point, in order to determine the mechanism underlying knee joint chondrocyte apoptosis. Apoptosis and ultrastructural changes in the articular cartilage were examined by pathological study and transmission electron microscopy, and PERK, eIF2α, and CHOP mRNA and protein levels were detected by qRT-PCR and western blot, respectively. PERK, eIF2α, and CHOP protein levels were significantly higher in the model group than in the normal group (P < 0.01) and were considerably downregulated in the needle knife and the medicine groups compared to the model group (P < 0.01). The eIF2α, p-eIF2α, and CHOP protein levels were not significantly different between the needle knife and medicine groups. The PERK, eIF2α, and CHOP mRNA levels in the drug group were higher than those in the needle knife group (P < 0.01). Needle knife therapy can regulate PERK-eIF2α-CHOP signaling pathway, which could be one of the mechanisms by which it affects chondrocyte apoptosis in KOA rabbits.