Vascular-derived artemin: a determinant of vascular sympathetic innervation?
Vascular-derived artemin: a determinant of vascular sympathetic innervation?
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DOI：
10.1152/ajpheart.00859.2006
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发表时间：
2007-07-01
影响因子：
4.8
通讯作者：
Marko, Stephen B.
中科院分区：
文献类型：
--
作者：
Damon, Deborah H.;teRiele, Jaclyn A.;Marko, Stephen B.
血管交感神经支配是血压和血流的重要决定因素。决定血管交感神经支配的机制尚不清楚。本研究验证了血管源性青蒿素通过促进交感神经轴突生长来促进交感神经对血管的发育的假说。RT-PCR和Western分析表明，青蒿素在培养的血管平滑肌和动脉中表达，青蒿素辅助受体--胶质细胞源性神经营养因子家族受体α3和ret在节后交感神经元中表达。在新生大鼠交感神经节外植体上观察青蒿素对轴突生长的影响。在没有神经生长因子的情况下，外源性青蒿素可刺激神经突起生长。成年大鼠股动脉含有青蒿素，这些动脉刺激交感神经突起生长。添加FA的生长量为92.2±11.9 mm，不添加FA的生长量为26.3±5.4 mm(P<；0.05)。FA对轴突生长的刺激作用可通过中和青蒿素活性的抗体来减少(P<；0.05)。这些数据表明，青蒿素在动脉中表达，其受体在支配动脉的节后交感神经元中表达并发挥功能。这提示青蒿素可能是血管交感神经支配的决定因素。
Vascular sympathetic innervation is an important determinant of blood pressure and blood flow. The mechanisms that determine vascular sympathetic innervation are not well understood. The present study tests the hypothesis that vascular-derived artemin promotes the development of sympathetic innervation to blood vessels by promoting sympathetic axon growth. RT-PCR and Western analyses indicate that artemin is expressed by cultured vascular smooth muscle and arteries, and artemin coreceptors, glial cell-derived neurotrophic factor family receptor alpha 3 and ret, are expressed by postganglionic sympathetic neurons. The effects of artemin on axon growth were assessed on explants of neonatal rat sympathetic ganglia. In the presence, but not in the absence, of nerve growth factor, exogenous artemin stimulated neurite growth. Femoral arteries (FA) from adult rats contain artemin, and these arteries stimulated sympathetic neurite growth. Growth in the presence of FA was 92.2 +/- 11.9 mm, and that in the absence of FA was 26.3 +/- 5.4 mm (P < 0.05). FA stimulation of axon growth was reduced by an antibody that neutralized the activity of artemin (P < 0.05). These data indicate that artemin is expressed in arteries, and its receptors are expressed and functional in the postganglionic sympathetic neurons that innervate them. This suggests that artemin may be a determinant of vascular sympathetic innervation.