The regulation of Streptococcus mutans glucan-binding protein A expression.
The regulation of Streptococcus mutans glucan-binding protein A expression.
复制标题
变形链球菌葡聚糖结合蛋白 A 表达的调节。
DOI：
10.1111/j.1574-6968.1997.tb12658.x
复制
发表时间：
1997
影响因子：
2.1
通讯作者：
Singh,RN
中科院分区：
文献类型：
--
作者：
Banas,JA;Potvin,HC;Singh,RN
帖前mutansGBP-A被假设为组成型表达，并有助于蔗糖依赖性的S.变异人为了研究GBP-A的表达，将编码氯霉素乙酰转移酶（CAT）的报告基因置于gbpA启动子的下游，并在与S.变异人GBP-A在厌氧和中性pH条件下表达最佳，并与最佳生长相关。向生长培养基中添加蔗糖并没有提高GBP-A的表达。
TheS. mutansGBP-A is hypothesized to be constitutively expressed and to contribute to the sucrose-dependent colonization ofS. mutans. To investigate GBP-A expression, a reporter gene encoding chloramphenicol acetyltransferase (CAT) was placed downstream of thegbpApromoter and CAT activity was measured under conditions that would be associated with the sucrose-dependent colonization ofS. mutans. Expression of GBP-A was optimal under anaerobiosis and neutral pH conditions, and correlated with optimal growth. The addition of sucrose to the growth medium did not elevate the expression of GBP-A.