The microbial communities in two apparently physically separated deep subsurface oil reservoirs show extensive DNA sequence similarities
The microbial communities in two apparently physically separated deep subsurface oil reservoirs show extensive DNA sequence similarities
复制标题
DOI：
10.1111/1462-2920.12181
复制
发表时间：
2014-02-01
影响因子：
5.1
通讯作者：
Valla, Svein
中科院分区：
文献类型：
--
作者：
Lewin, Anna;Johansen, Jostein;Valla, Svein
众所周知，微生物在各种极端环境中定居，包括地壳深处的油藏等栖息地。在这里，我们提出了来自两个不同油藏的宏基因组的比较高覆盖率DNA测序研究的结果，这两个油藏都位于挪威海以下约2.5公里的海底。以前报道的生物信息学分析的DNA序列数据来自一个水库（井I）表明，该社区是由细菌物种占主导地位的一个较小的分数ofpectia。在此，我们报告了对位于同一地理区域的另一个油藏（II井）进行的类似分析结果，然而，根据现有的地质知识，该油藏与I井没有直接的物理接触。有趣的是，井II社区主要由细菌占主导地位，细菌占次要地位。两个数据集的比较表明，大部分序列在同一性（通常高于98%）和基因组织方面都非常相似。因此，我们得出结论，两个威尔斯孔基本上含有相同的生物体，但相对丰度不同。假设由于物理分离，这些群落在很长一段时间内都是不同的，结果还表明，水库中的微生物生长非常缓慢。
It is well established that micro‐organisms colonize a variety of extreme environments, including habitats like oil reservoirs deep inside the earth crust. Here, we present the results of a comparative high‐coverage DNA sequencing study of metagenomes derived from two different oil reservoirs, both located about 2.5 km subseafloor below the Norwegian Sea. A previously reported bioinformatic analysis of DNA sequence data derived from one of the reservoirs (Well I) indicated that the community is dominated by bacterial species with a smaller fraction ofArchaea. Here, we report results of a similar analysis from another reservoir (Well II) located in the same geographical area, however, according to available geological knowledge lacking direct physical contact with Well I. Interestingly, the Well II community is largely dominated byArchaeawith a subordinate fraction ofBacteria. Comparison of the two datasets showed that large fractions of the sequences are extremely similar, both with respect to identity (typically above 98%) and gene organization. We therefore conclude that both wells contain essentially the same organisms, but in different relative abundances. Assuming that the communities have been distinct for long timescales because of physical separation, the results also indicate that microbial growth in the reservoirs is extremely slow.