Phylogenetic analysis identifies many uncharacterized actin-like proteins (Alps) in bacteria: regulated polymerization, dynamic instability and treadmilling in Alp7A.
Phylogenetic analysis identifies many uncharacterized actin-like proteins (Alps) in bacteria: regulated polymerization, dynamic instability and treadmilling in Alp7A.
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DOI：
10.1111/j.1365-2958.2009.06771.x
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发表时间：
2009-08
影响因子：
3.6
通讯作者：
Pogliano J
中科院分区：
文献类型：
--
作者：
Derman AI;Becker EC;Truong BD;Fujioka A;Tucey TM;Erb ML;Patterson PC;Pogliano J
Actin, one of the most abundant proteins in the eukaryotic cell, also has an abundance of relatives in the eukaryotic proteome. To date though, only five families of actins have been characterized in bacteria. We have conducted a phylogenetic search and uncovered more than 35 highly divergent families of actin-like proteins (Alps) in bacteria. Their genes are found primarily on phage genomes, on plasmids, and on integrating conjugative elements, and are likely to be involved in a variety of functions. We characterize three Alps and find that all form filaments in the cell. The filaments of Alp7A, a plasmid partitioning protein and one of the most divergent of the Alps, display dynamic instability and also treadmill. Alp7A requires other elements from the plasmid to assemble into dynamic polymers in the cell. Our findings suggest that most if not all of the Alps are indeed actin relatives, and that actin is very well represented in bacteria.
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影响因子：
9.8
作者：
Dayel MJ;Mullins RD
通讯作者：
Mullins RD
影响因子：
9.2
作者：
Egelman, EH
通讯作者：
Egelman, EH
影响因子：
3.5
作者：
GueroutFleury, AM;Frandsen, N;Stragier, P
通讯作者：
Stragier, P
影响因子：
7.8
作者：
Campbell, Christopher S.;Mullins, R. Dyche
通讯作者：
Mullins, R. Dyche
DOI：
10.1073/pnas.0402638101
发表时间：
2004-06-08
影响因子：
11.1
作者：
Gitai, Z;Dye, N;Shapiro, L
通讯作者：
Shapiro, L