Novel potato C2H2-type zinc finger protein gene, StZFP1, which responds to biotic and abiotic stress, plays a role in salt tolerance.
Novel potato C2H2-type zinc finger protein gene, StZFP1, which responds to biotic and abiotic stress, plays a role in salt tolerance.
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DOI：
10.1111/j.1438-8677.2009.00276.x
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发表时间：
2010-09
期刊：
影响因子：
3.9
通讯作者：
Zhendong Tian;Y. Zhang;Jian Liu;C. Xie
中科院分区：
文献类型：
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作者：
Zhendong Tian;Y. Zhang;Jian Liu;C. Xie
许多tfiia型锌指蛋白（ZFPs）在植物的逆境响应中起着重要作用。本研究从马铃薯中克隆了一个新的锌指蛋白基因StZFP1。StZFP1是典型的tfiia型双指锌指基因，具有1个B-box结构域、1个L-box结构域和1个DLN-box/EAR基序。StZFP1基因属于一个小的基因家族，估计拷贝数为4或5，位于1号染色体上，StZFP1在成体植物的叶、茎、根、块茎和花中都有组成性表达。盐、脱水和外源ABA均可诱导StZFP1的表达。StZFP1的表达也对晚疫病病原菌疫霉的感染有反应。StZFP1:GFP融合蛋白的瞬时表达分析显示，StZFP1优先定位于细胞核。在拟南芥rd29A启动子的驱动下，StZFP1在转基因烟草中的异位表达提高了植物对盐胁迫的耐受性。这些结果表明StZFP1可能通过aba依赖途径参与马铃薯对盐和脱水胁迫的反应。
Many TFIIIA-type zinc finger proteins (ZFPs) play important roles in stress responses in plants. In the present study, a novel zinc finger protein gene, StZFP1, was cloned from potato. StZFP1 is a typical TFIIIA-type two-finger zinc finger gene with one B-box domain, one L-box domain and a DLN-box/EAR motif. The StZFP1 genes belong to a small gene family with an estimated copy number of four or five, located on chromosome I. StZFP1 is constitutively expressed in leaves, stems, roots, tubers and flowers of adult plants. Expression of StZFP1 can be induced by salt, dehydration and exogenously applied ABA. StZFP1 expression is also responsive to infection by the late blight pathogen Phytophthora infestans. Transient expression analysis of StZFP1:GFP fusion protein revealed that StZFP1 is preferentially localised in the nucleus. Ectopic expression of StZFP1, driven by the Arabidopsis rd29A promoter in transgenic tobacco, increased plant tolerance to salt stress. These results demonstrate that StZFP1 might be involved in potato responses to salt and dehydration stresses through an ABA-dependent pathway.