THE GROWTH OF LARGE SINGLE-CRYSTALS OF LYSOZYME
THE GROWTH OF LARGE SINGLE-CRYSTALS OF LYSOZYME
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DOI：
10.1002/bip.360250213
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发表时间：
1986-02-01
期刊：
影响因子：
2.9
通讯作者：
TANAKA, S
中科院分区：
文献类型：
--
作者：
ATAKA, M;TANAKA, S
如果蛋白质单晶大于那些适合于X射线分析获得各种光谱，热，机械和电气测量成为可能。为了理解控制晶体尺寸的因素，在15 ℃下分批生长四氢溶菌酶晶体。C从不同的蛋白质和盐浓度之间的pH 4-7的溶液。晶体的数量和大小以及保留在上清液中的蛋白质浓度随初始盐量、pH和阳离子种类而显著变化，但是当初始蛋白质浓度（P）在晶体溶解度（S）的2.5-3倍的窄范围内时，大晶体总是生长。（1）第一次晶体出现前的时间（D）随D的变化而变化。（P/S）-n，其中N. 5，和（2），许多以前的实验使用更多的过饱和溶液比最佳的，从而确定。讨论了大晶体只能从中等过饱和溶液中生长的原因。在40 ° C下生长的斜方晶型的晶体尺寸。C对pH和P的敏感性低于四环素形式。尝试测量D和在40 ℃下的溶解度。C揭示了两种晶型之间的许多差异，我们将其归因于促进结晶的不同相互作用。
If protein single crystals larger than those suitable for X-ray analysis are obtained various spectroscopic, thermal, mechanical, and electrical measurements become possible. To understand the factors governing the crystal size, tetragonal lysozyme crystals were grown in batches at 15.degree. C from solutions of different protein and salt concentrations between pH 4-7. The number and size of the crystals, and the protein concentration remaining in the supernatant, varied markedly with the initial salt amount, pH, and cation species, but large crystals always grew when the initial protein concentration (P) was in a narrow range of 2.5-3 times the crystal solubility (S). It was also shown (1) that the period before the first crystals appeared (D) varied as D .varies. (P/S)-n, where N .simeq. 5, and (2) that many previous experiments used more supersaturated solutions than the optimal ones thus determined. The reason why large crystals grow only from moderately supersaturated solutions is discussed. The crystal size of the orthorhombic form grown at 40.degree. C was less sensitive to pH and P than the tetragonal form. An effort to measure D and the solubility at 40.degree. C revealed many differences between the two crystal forms, which we ascribe to different interactions to promote crystallization.