Genogroup I picobirnaviruses in pigs:: evidence for genetic diversity and relatedness to human strains
Genogroup I picobirnaviruses in pigs:: evidence for genetic diversity and relatedness to human strains
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DOI：
10.1099/vir.0.83134-0
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发表时间：
2008-02-01
影响因子：
3.8
通讯作者：
Szucs, G.
中科院分区：
文献类型：
--
作者：
Banyai, K.;Martella, V.;Szucs, G.
微小RNA病毒（PBV）是一种小型无包膜病毒，具有双片段双链RNA基因组。它们的致病潜力、生态学和进化特征在很大程度上未被探索。在这里，我们描述了在死猪的肠道内容物中鉴定的猪PBV的分子分析。对13份阳性样品中的6份进行了克隆，并进行了单链构象多态性分析和核苷酸序列测定。所有克隆都属于基因组I PBV，并且几乎所有克隆都聚集在来自人毒株的单独分支上。单个菌株与匈牙利人PBV株（89.9nt和96.4%aa同一性）具有显著密切的遗传关系。在混合感染中鉴定的菌株之间也观察到遗传多样性。单点突变和高度相关的菌株内的有害突变表明，PBV存在准种在猪消化道。来自不同动物的具有完整序列同一性的克隆表明该病毒在动物间的有效传播。我们的研究结果表明，感染基因组I PBV是常见的猪。
Picobirnaviruses (PBVs) are small, non-enveloped viruses with a bisegmented double-stranded RNA genome. Their pathogenic potential, ecology, and evolutionary features are largely unexplored. Here, we describe the molecular analysis of porcine PBVs identified in the intestinal content of dead pigs. Six of 13 positive samples were cloned and then subjected to single-strand conformation polymorphism analysis and nucleotide sequencing. All clones belonged to genogroup I PBVs and almost all clones clustered on separate branches from human strains. A single strain shared a notably close genetic relationship with a Hungarian human PBV strain (89.9 nt and 96.4% aa identity). Genetic diversity was also observed among strains identified in mixed infections. Single point mutations and deleterious mutations within highly related strains suggested that PBVs exist as quasispecies in the swine alimentary tract. Clones with complete sequence identities originating from different animals suggested effective animal-to-animal transmission of the virus. Our findings indicate that infection with genogroup I PBVs is common in pigs.