Differences between the estradiol and testosterone receptors in rat uterus.
Differences between the estradiol and testosterone receptors in rat uterus.
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大鼠子宫中雌二醇和睾酮受体之间的差异。
DOI：
10.1111/j.1432-1033.1974.tb03791.x
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
F. Lignon
中科院分区：
文献类型：
--
作者：
H. Rochefort;F. Lignon
未成熟的大鼠子宫含有雌二醇和睾酮的特异结合蛋白。为了比较这两个荷尔蒙部位并确定它们的定位，先后建立了以下实验。
1
睾酮不与[~3H]雌二醇(3 NM)竞争雌激素受体部位，而高浓度的雌二醇与[~3H]睾酮在雄激素受体上竞争。
2.
在低生理浓度的睾酮作用下孵育子宫后，大多数睾酮受体定位于细胞核，而雌二醇受体定位于胞浆部分。对于较高浓度的睾酮(≥100 nM)，两个类固醇受体部位主要出现在核部分。
3.
同时制备和分析的[~3H]睾酮和[~(14)C]雌二醇与胞浆受体的络合物，在蔗糖梯度超速离心和琼脂糖层析后表现出不同的表现。
这些结果有力地表明，大鼠子宫中睾酮和雌二醇的受体位置定位于两个不同的多单位蛋白质上，其中类固醇结合单位不同。
Immature rat uteri contain specific binding proteins for both estradiol and testosterone. In an attempt to compare these two hormonal sites and to determine their localisation, the following was successively established.
1
Testosterone did not compete with [3H]estradiol (3 nM) for the estrogen receptor sites, whereas high concentrations of estradiol did compete with [3H]testosterone on the androgen receptor.
2
After incubation of uteri at low physiological concentration of testosterone, the majority of the testosterone receptor sites was localised in the nucleus, whereas the estradiol receptor sites were found in the cytosol fraction. For higher concentration (≥ 100 nM) of testosterone the two steroid receptor sites were mainly present in the nuclear fraction.
3
The complexes of [3H]testosterone and [14C]estradiol with cytosol receptors, when simultaneously prepared and analysed, were shown to behave differently after sucrose-gradient ultracentrifugation and Sepharose chromatography.
These results strongly suggest that the receptor sites for testosterone and estradiol in rat uterus are localised on two different multi-unit proteins in which the steroid binding units are different.