The eIF4E-binding protein Eap1 has similar but independent roles in cell growth and gene expression with the cytoplasmic deadenylase Ccr4
The eIF4E-binding protein Eap1 has similar but independent roles in cell growth and gene expression with the cytoplasmic deadenylase Ccr4
复制标题
eIF4E 结合蛋白 Eap1 在细胞生长和基因表达中与细胞质去腺苷酸酶 Ccr4 具有相似但独立的作用
DOI：
10.1093/bbb/zbab056
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Irie Kenji
中科院分区：
文献类型：
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作者：
Higuchi Yudai;Fujii Shiori;Valderrama Arvin Lapiz;Irie Kaoru;Suda Yasuyuki;Mizuno Tomoaki;Irie Kenji
eIF 4 E结合蛋白（4 E-BP）是与eIF 4G竞争结合eIF 4 E的翻译阻遏物。在这里，我们研究了酵母4 E-BPs，Eap 1和Caf 20在细胞壁完整性途径和基因表达中的作用。我们发现，p1 α突变，而不是caf 20 α突变，表现为合成生长缺陷，并在ROM 2基因编码Rho 1 GEF突变。eap 1突变也表现出合成致死性，编码胞质去腺苷酶的CCR 4基因突变。Ccr 4参与了编码Rho 1 GAP的LRG 1 mRNA的降解。Eap 1-Y109 A L114 A不能与eIF 4 E结合，但不能抑制Eap 1 α ccr 4 β突变体的合成致死性，表明Eap 1的4 E结合对其功能很重要。我们还发现ap 1突变体表现出应激反应基因HSP 12的去抑制。Eap 1的4 E结合也是抑制HSP 12表达所必需的。我们的研究结果表明，Eap 1在细胞生长和基因表达中与Ccr 4具有相似但独立的作用。
eIF4E-binding proteins (4E-BPs) are translational repressors that compete with eIF4G for binding to eIF4E. Here we investigated the roles of yeast 4E-BPs, Eap1, and Caf20 in cell wall integrity pathway and gene expression. We found thateap1∆mutation, but notcaf20∆mutation, showed synthetic growth defect with mutation inROM2gene encoding Rho1 GEF. Theeap1∆mutation also showed synthetic lethality with mutation inCCR4gene encoding cytoplasmic deadenylase. Ccr4 functions in the degradation ofLRG1mRNA encoding Rho1 GAP. Eap1-Y109A L114A, which could not bind to eIF4E, did not suppress the synthetic lethality ofeap1∆ ccr4∆mutant, suggesting that 4E-binding of Eap1 is important for its function. We also found thateap1∆mutant showed the derepression of stress response geneHSP12. 4E-binding of Eap1 was also required for the repression ofHSP12expression. Our results indicate that Eap1 has similar but independent roles in cell growth and gene expression with Ccr4.