Multiple-metal exposure, diet, and oxidative stress in Uruguayan school children.
Multiple-metal exposure, diet, and oxidative stress in Uruguayan school children.
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乌拉圭学童的多种金属暴露、饮食和氧化应激。
DOI：
10.1016/j.envres.2018.06.028
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发表时间：
2018
影响因子：
8.3
通讯作者：
Queirolo,ElenaI
中科院分区：
文献类型：
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作者：
Kordas,Katarzyna;Roy,Aditi;Vahter,Marie;Ravenscroft,Julia;Mañay,Nelly;Peregalli,Fabiana;Martínez,Gabriela;Queirolo,ElenaI
氧化应激（OS）是接触有毒金属的重要后果，但目前尚不清楚低水平金属暴露在多大程度上有助于儿童OS。我们研究了尿砷（As）、镉（Cd）和铅（Pb）浓度与OS的尿标志物：F2-8α异前列腺素和8-羟基-2-脱氧鸟苷（8-OHdG）之间的横断面相关性。我们还测试了饮食摄入量的影响。在211名6-8岁的蒙得维的亚儿童中，有143名儿童被纳入完整病例分析，他们有资格参加这项研究，因为他们至少有一个OS标志物通过ELISA测量。采用电感耦合等离子体质谱法（ICP-MS：Pb、Cd）和高效液相色谱-氢化物发生ICP-MS（As-代谢物）在线测定尿金属;浓度经对数转换。根据尿比重调整所有尿液标志物。进行了两次24小时的膳食回顾，以估计儿童的膳食摄入量，包括水果和蔬菜的总摄入量以及维生素C、锌和纤维的摄入量。普通最小二乘法（OLS）和加权分位数和（WQS）回归用于估计金属和每个OS标志物之间的相关性作为结果。金属暴露水平普遍较低：尿砷、镉、铅中位数分别为9.6 μg/L、0.06 μg/L和1.9 μg/L。中位8-异前列烷浓度为1.1 ng/mL，8-OHdG为39.6 ng/mL。在协变量调整的OLS模型中，Log 2转换的尿As浓度与8-OHdG浓度呈正相关（10.90 [3.82，17.97]），该模型还考虑了Cd和Pb暴露。在WQS中，混合物指数也与较高的8-OHdG相关（指数值每增加25%为8.71 [1.12，16.3]），主要由As暴露驱动。几乎没有证据表明膳食抗氧化剂的影响修改。总之，即使在低水平下，As暴露也与可检测到的DNA氧化损伤有关。
Oxidative stress (OS) is an important consequence of exposure to toxic metals but it is unclear to what extent low-level metal exposures contribute to OS in children. We examined the cross-sectional association between urinary concentrations of arsenic (As), cadmium (Cd), and lead (Pb) and urinary markers of OS: F2–8α isoprostane and 8-hydroxy-2-deoxy-guanosine (8-OHdG). We also tested effect modification by dietary intakes. Of the 211 children aged 6–8 years living in Montevideo who were eligible for the study because they had at least one OS marker measured via ELISA, 143 were included in a complete-case analysis. Urinary metals were measured with inductively coupled plasma mass spectrometry (ICP-MS: Pb, Cd) and high-performance liquid chromatography online with hydride generation ICP-MS (As-metabolites); concentrations were log2-transformed. All urinary markers were adjusted for specific gravity of urine. Two 24-h dietary recalls were conducted to estimate children's dietary intakes, including total fruit and vegetable consumption and vitamin C, zinc and fiber intake. Ordinary least square (OLS) and weighted quantile sum (WQS) regressions were used to estimate the association between metals and each OS marker as outcome. Metal exposure was generally low: median urinary As, Cd, Pb 9.6 μg/L, 0.06 μg/L and 1.9 μg/L, respectively. Median 8-isoprostane concentration was 1.1 and 8-OHdG 39.6 ng/mL. Log2-transformed urinary As concentrations were positively associated with 8-OHdG concentrations (10.90 [3.82, 17.97]) in covariate-adjusted OLS models which also took account of exposure to Cd and Pb. In WQS, a mixture index was also associated with higher 8-OHdG (8.71 [1.12, 16.3] for each 25% increase in index value), mostly driven by As exposure. There was little evidence of effect modification by dietary antioxidants. In sum, even at low-level, As exposure is associated with detectable oxidative damage to the DNA.